PE-Labeled Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Tetramer Protein

Synonyms

HLA-A*0201 & B2M & CMV pp65 (NLVPMVATV)

Source

PE-Labeled Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Tetramer Protein (HLC-HP2H6) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & NLVPMVATV peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769 (B2M) & NLVPMVATV).

Predicted N-terminus: Gly 25 & Ile 21

Molecular Characterization

PE-Labeled Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Tetramer Protein is assembled by biotinylated monomer (HLC-H82E5) and PE-labeled streptavidin.

Biotinylated Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Complex Protein is produced by co-expression of HLA and B2M loaded with CMV pp65 peptide. Biotinylated Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

Conjugate

PE

Excitation Wavelength: 488 nm / 561 nm

Emission Wavelength: 575 nm

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, 1% BSA, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

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