Biotinylated Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His,Avitag™ (MALS & SPR verified)

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製品番号/仕様
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数量
HNN-M82E3-200ug
$1520.00
HNN-M82E3-1mg (200ug X 5)
$5080.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    HN protein

  • Source

    Biotinylated Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His,Avitag (HNN-M82E3) is expressed from human 293 cells (HEK293). It contains AA Gly 106 - Thr 582 (Accession # P11235).

    Predicted N-terminus: Gly 106

    Request for sequence
  • Molecular Characterization

    HN protein Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 56.3 kDa. The protein migrates as 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    HN protein SDS-PAGE

    Biotinylated Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    HN protein SEC-MALS

    The purity of Biotinylated Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His,Avitag (Cat. No. HNN-M82E3) is more than 85% and the molecular weight of this protein is around 60-85 kDa verified by SEC-MALS.

    Report
  • Bioactivity-SPR

     HN protein SPR

    Biotinylated Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His,Avitag (Cat. No. HNN-M82E3) immobilized on CM5 Chip can bind e 3'-Sialyllactose / GM3 Ganglioside oligosaccharide with an affinity constant of 222 μM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol

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バックグランド

The two surface glycoproteins of the mumps virus are the hemagglutinin-neuraminidase (HN) and Fusion proteins. These glycoproteins are essential for viral entry to host cells, and the spread of newly formed virions. The HN protein is a 582-amino acid structural glycoprotein of the mumps virus. HN is a type II membrane protein in which the N terminus is oriented towards the cytoplasm and the C terminus is extracellular.The HN protein exhibits both hemagglutinin and neuraminidase properties and is critical for membrane fusion and viral entry into host cells.

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  • 英語名:

    Hemagglutinin-neuraminidase

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