製品詳細
Synonyms
HN protein
Source
Biotinylated Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His,Avitag (HNN-M82E3) is expressed from human 293 cells (HEK293). It contains AA Gly 106 - Thr 582 (Accession # P11235).
Predicted N-terminus: Gly 106
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 56.3 kDa. The protein migrates as 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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データ表示
SDS-PAGE

Biotinylated Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Biotinylated Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His,Avitag (Cat. No. HNN-M82E3) is more than 85% and the molecular weight of this protein is around 60-85 kDa verified by SEC-MALS.
Report
Bioactivity-SPR

Biotinylated Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His,Avitag (Cat. No. HNN-M82E3) immobilized on CM5 Chip can bind e 3'-Sialyllactose / GM3 Ganglioside oligosaccharide with an affinity constant of 222 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
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最新の進展
- 英語名:
Hemagglutinin-neuraminidase
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