製品詳細
Synonyms
IFN-alpha 1, IFNA1, IFNalpha 1, IFN-alpha-1, LeIF D
Source
Human IFN-alpha 1, His Tag (IFA-H52H9) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Glu 189 (Accession # P01562-1).
Predicted N-terminus: Cys 24
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 21.3 kDa. The protein migrates as 22-24 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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データ表示
SDS-PAGE

Human IFN-alpha 1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-BLI

Loaded Human IFN-alpha 1, His Tag (Cat. No. IFA-H52H9) on HIS1K Biosensor, can bind Human IFNAR1, Fc Tag (Cat. No. IF1-H5253) with an affinity constant of 0.191 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human IFN-alpha 1, His Tag (Cat. No. IFA-H52H9) on HIS1K Biosensor, can bind Human IFNAR2, Fc Tag (Cat. No. IF2-H5255) with an affinity constant of 19.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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