Biotinylated Mouse IFN-gamma Protein, His,Avitag™ (MALS verified)

Customer Reviews
Cat. No. / Size
Price
Qty
IFG-M82E3-25ug
$315.00
IFG-M82E3-200ug
$1280.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    Interferon-gamma , Interferon-γ

  • Source

    Biotinylated Mouse IFN-gamma Protein, His,Avitag (IFG-M82E3) is expressed from human 293 cells (HEK293). It contains AA His 23 - Cys 155 (Accession # P01580).

    Predicted N-terminus: His 23

    Request for sequence
  • Molecular Characterization

    IFN-gamma Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 19.1 kDa. The protein migrates as 20-25 kDa and 26-31 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

    The protein is designed as a dimer.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

Customers Also Viewed

Performance Data

  • SDS-PAGE

    IFN-gamma SDS-PAGE

    Biotinylated Mouse IFN-gamma Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IFN-gamma SEC-MALS

    The purity of Biotinylated Mouse IFN-gamma Protein, His,Avitag (Cat. No. IFG-M82E3) is more than 85% and the molecular weight of this protein is around 50-65 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     IFN-gamma ELISA

    Immobilized Biotinylated Mouse IFN-gamma Protein, His,Avitag (Cat. No. IFG-M82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Rabbit Anti-Mouse IFN-gamma antibody with a linear range of 0.02-1 ng/mL (QC tested).

    Protocol

Customer Reviews
Writing Reviews

Background

Interferon-gamma (IFN-γ/IFNG) is a dimerized soluble cytokine that is the only member of the type II class of interferon. This interferon was originally called macrophage-activating factor, a term now used to describe a larger family of proteins to which IFN-γ belongs. IFN-gamma has been used in a wide variety of clinical indications. Interferon-gamma (IFNgamma) is a central regulator of the immune response and signals via the Janus Activated Kinase (JAK)-Signal Transducer and Activator of Transcription (STAT) pathway. Interferon gamma has broader roles in activation of innate and adaptive immune responses to viruses and tumors, in part through upregulating transcription of genes involved in cell cycle regulation, apoptosis, and antigen processing/presentation. Despite this, rodent and human trophoblast cells show dampened responses to IFNG that reflect the resistance of these cells to IFNG-mediated activation of major histocompatibility complex (MHC) class II transplantation antigen expression.

Recent Advances

 
Drug Development Progress
  • English Name:

    Interferon gamma

  • Category:

  • Approved Drugs:

    4 Details

  • Drugs in Clinical Trials:

    14 Details

  • Highest Development Stage:

    Approved

Contact Us
Drug Candidate Licensing
More
  • Basic Product Information
  • Product Details
  • Customers Also Viewed
  • Performance Data
  • Customer Reviews
  • Background
Contact Us

お問い合わせ
グローバル:+1 800-810-0816(Toll Free)

メールアドレス
order.jp@acrobiosystems.com

アクロバイオシステムズ株式会社
〒101-0051
東京都千代田区神田神保町1-32-8