Biotinylated Human IGF-II Protein, Avitag™,Fc Tag

Synonyms

IGF2, C11orf43, FLJ22066, FLJ44734, IGF-II, PP9974

Source

Biotinylated Human IGF-II, Avitag,Fc Tag (IG2-H82F9) is expressed from human 293 cells (HEK293). It contains AA Ala 25 - Glu 91 (Accession # P01344-1).

Predicted N-terminus: Gly

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a human IgG1 Fc tag.

The protein has a calculated MW of 35.3 kDa. The protein migrates as 38-42kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Protein Ratio

Passed as determined by the HABA assay / binding ELISA.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process