Human IGFBP-1 Protein, His Tag (MALS verified)

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製品番号/仕様
価格
数量
IG1-H52H3-100ug
$310.00
IG1-H52H3-1mg (500ug X 2)
$2040.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    IGFBP1, PP12, IBP1

  • Source

    Human IGFBP-1 Protein, His Tag (IG1-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Asn 259 (Accession # P08833).

    Predicted N-terminus: Ala 26

    Request for sequence
  • Molecular Characterization

    IGFBP-1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 27.2 kDa. The protein migrates as 32-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    IGFBP-1 SDS-PAGE

    Human IGFBP-1 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IGFBP-1 SEC-MALS

    The purity of Human IGFBP-1 Protein, His Tag (Cat. No. IG1-H52H3) is more than 95% and the molecular weight of this protein is around 22-32 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     IGFBP-1 ELISA

    Immobilized Human IGF-I, Fc Tag at 5 μg/mL (100 μL/well) can bind Human IGFBP-1 Protein, His Tag (Cat. No. IG1-H52H3) with a linear range of 0.01-0.156 μg/mL (QC tested).

    Protocol

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バックグランド

Insulin-like growth factor-binding protein 1 (IGFBP1) is also known as placental protein 12 (PP12), which contains oneIGFBP N-terminal domain and one thyroglobulin type-1 domain. IGFBP1 can bindd both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of IGFBP1 can prolongd the half-life of the IGFs and alterd their interaction with cell surface receptors. Furthermore, IGFBP1 can promote cell migration. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

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