Human IL-1 alpha / IL-1F1 Protein, His Tag, low endotoxin (MALS verified)

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製品番号/仕様
価格
数量
ILA-H5216-100ug
$395.00
ILA-H5216-1mg
$2615.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    IL1A, IL-1 ALPHA, IL1F1, IL1α

  • Source

    Human IL-1 alpha, His Tag (ILA-H5216) is expressed from human 293 cells (HEK293). It contains AA Ser 113 - Ala 271 (Accession # P01583-1).

    Predicted N-terminus: Ser 113

    Request for sequence
  • Molecular Characterization

    IL-1 alpha Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 19.9 kDa. The protein migrates as 25-27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    IL-1 alpha SDS-PAGE

    Human IL-1 alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    IL-1 alpha SEC-MALS

    The purity of Human IL-1 alpha, His Tag (Cat. No. ILA-H5216) is more than 95% and the molecular weight of this protein is around 20-30 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     IL-1 alpha ELISA

    Immobilized Human IL-1 RI Protein, Fc Tag (Cat. No. ILI-H5253) at 10 μg/mL (100 μL/well) can bind Human IL-1 alpha, His Tag (Cat. No. ILA-H5216) with a linear range of 0.3-2.5 μg/mL (QC tested).

    Protocol

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バックグランド

Interleukin-1 alpha (IL1a) is also known as IL-1A, IL1, IL1-ALPHA,IL1F1,and is a cytokine of the interleukin-1 family. IL1a possesses a wide spectrum of metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. IL-1α is constitutively produced by epithelial cells. It is found in substantial amounts in normal human epidermis and is distributed in a 1:1 ratio between living epidermal cells and stratum corneum.The constitutive production of large amounts of IL-1α precursor by healthy epidermal keratinocytes interfere with the important role of IL-1α in immune responses, assuming skin as a barrier, which prevents the entry of pathogenic microorganisms into the body. In vitro, IL-1α possesses biological effect on cells in the picomolar to femtomolar range. In vivo, Shortly after an onset of an infection into organism, IL-1α activates a set of immune system response processes.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Interleukin-1 alpha

  • カテゴリ:

  • 市販の医薬品数:

    1 詳細

  • 治験医薬品数:

    11 詳細

  • 最新の開発ステージ:

    Approved

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