Mouse IL-17A / CTLA-8 Protein, His Tag, low endotoxin (MALS verified)

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製品番号/仕様
価格
数量
CT8-M5240-100ug
$435.00
CT8-M5240-1mg (250ug X 4)
$3060.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    IL-17A, Interleukin-17A, CTLA-8, IL-17

  • Source

    Mouse IL-17A, His Tag (CT8-M5240) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Ala 158 (Accession # Q62386-1).

    Predicted N-terminus: His

    Request for sequence
  • Molecular Characterization

    IL-17A Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 15.8 kDa. The protein migrates as 18 kDa, 20-23 kDa, 26 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

    The protein is designed as a dimer.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    IL-17A SDS-PAGE

    Mouse IL-17A, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IL-17A SEC-MALS

    The purity of Mouse IL-17A, His Tag (Cat. No. CT8-M5240) is more than 85% and the molecular weight of this protein is around 30-50 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     IL-17A ELISA

    Immobilized Mouse IL-17A, His Tag (Cat. No. CT8-M5240) at 2 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tag with a linear range of 4-125 ng/mL (QC tested).

    Protocol
  • Bioactivity-BLI

     IL-17A BLI

    Loaded Human IL-17 RA, Fc Tag on Protein A Biosensor, can bind Mouse IL-17A, His Tag (Cat. No. CT8-M5240) with an affinity constant of 20.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

    Protocol
  • Bioactivity-CELL BASE

     IL-17A CELL

    Mouse IL-17A, His Tag (Cat. No. CT8-M5240) stimulates secretion of IL-6 by NIH-3T3 cells. The ED50 for this effect is 2.132-5.005 ng/mL.

    Protocol

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バックグランド

Interleukin-17A (IL17A) is also known as cytotoxic T-lymphocyte-associated antigen 8 (CTLA8),which is a proinflammatory cytokine produced by activated T cells. IL17A can regulate the activities of NF-kappaB and mitogen-activated protein kinases. Also,IL17A can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO).Furthermore,IL17A has been found both in glycosylated and nonglycosylated forms. High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Interleukin 17A

  • カテゴリ:

  • 市販の医薬品数:

    5 詳細

  • 治験医薬品数:

    35 詳細

  • 最新の開発ステージ:

    Approved

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