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Product Details
Synonyms
IL-17F, Interleukin-17F, Cytokine ML-1
Source
Human IL-17F, His Tag (ILF-H5244) is expressed from human 293 cells (HEK293). It contains AA Arg 31 - Gln 163 (Accession # Q96PD4-1).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 16.8 kDa. The protein migrates as 22-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human IL-17F, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human IL-17F, His Tag (Cat. No. ILF-H5244) is more than 95% and the molecular weight of this protein is around 38-48 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human IL-17F, His Tag (Cat. No. ILF-H5244) at 5 μg/mL (100 μL/well) can bind Human IL-17 RA Protein, Fc Tag (Cat. No. ILA-H525f) with a linear range of 2-39 ng/mL (QC tested).
Protocol
Immobilized Human IL-17F, His Tag (Cat. No. ILF-H5244) at 2 μg/mL (100 μL/well) can bind Human IL-17 RC, Fc Tag with a linear range of 0.016-0.25 μg/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human IL-17 RA, Fc Tag on Protein A Biosensor, can bind Human IL-17F, His Tag (Cat. No. ILF-H5244) with an affinity constant of 219 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human IL17RA & IL17RC Protein, Fc Tag&Fc Tag (Cat. No. ILC-H5257) on Protein A Biosensor, can bind Human IL-17F, His Tag (Cat. No. ILF-H5244) with an affinity constant of 1.04 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human IL-17F, His Tag (Cat. No. ILF-H5244) on NTA Biosensor, can bind Human IL-17 RC, Fc Tag with an affinity constant of 100 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Interleukin-17F
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
5 Details
- Highest Development Stage：
NDA/BLA
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