Human IL-18BP Protein, His Tag, low endotoxin (MALS & SPR verified)

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製品番号/仕様
価格
数量
ILP-H5222-200ug
$260.00
ILP-H5222-1mg (500ug X 2)
$1055.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    IL18BP, IL18BPa, Tadekinig-alfa

  • Source

    Human IL-18BP, His Tag (ILP-H5222) is expressed from human 293 cells (HEK293). It contains AA Thr 31 - Gly 194 (Accession # NP_001138529).

    Predicted N-terminus: Thr 31

    Request for sequence
  • Molecular Characterization

    IL-18BP Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 18.5 kDa. The protein migrates as 35-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    IL-18BP SDS-PAGE

    Human IL-18BP, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IL-18BP SEC-MALS

    The purity of Human IL-18BP, His Tag (Cat. No. ILP-H5222) is more than 95% and the molecular weight of this protein is around 32-45 kDa verified by SEC-MALS.

    Report
  • Bioactivity-SPR

     IL-18BP SPR

    Human IL-18 Protein, premium grade (Cat. No. IL8-H5114) immobilized on CM5 Chip can bind Human IL-18BP, His Tag (Cat. No. ILP-H5222) with an affinity constant of 0.319 nM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol
  • Bioactivity-CELL BASE

     IL-18BP CELL

    Human IL-18BP, His Tag (Cat. No. ILP-H5222) inhibits the IL-18-induced IFN-gamma secretion by KG‑1 cells. The ED50 for this effect is 0.019-0.053 µg/mL in the presence of 30 ng/mL of Recombinant Human IL‑18 (Routinely tested).

    Protocol

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バックグランド

Interleukin-18-binding protein (IL18BP) is also known as Tadekinig-alfa, IL18BPa, which functions as an IL18 inhibitor. IL18BP is a secreted protein which contains one Ig-like C2-type (immunoglobulin-like) domain. IL18BP does not have a transmembrane domain and hence is not anchored to the cell membrane. IL18BP is strongly expressed in heart, lung, placenta and spleen. Isoform A of IL18BP binds to IL-18 and inhibits its activity. IL18BP also functions as an inhibitor of the early TH1 cytokine response. IL18BP suppresses the production of IFN-gamma resulting in reduced T-helper type 1 immune responses.

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