Cynomolgus IL-2 R alpha / CD25 Protein, His Tag, low endotoxin (MALS verified)

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製品番号/仕様
価格
数量
ILA-C52H8-100ug
$310.00
ILA-C52H8-1mg (500ug X 2)
$2050.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    IL2RA, CD25, p55, IL2-RA, IL-2-RA

  • Source

    Cynomolgus IL-2 R alpha Protein, His Tag (ILA-C52H8) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Glu 238 (Accession # H6WS54).

    Predicted N-terminus: Glu 22

    Request for sequence
  • Molecular Characterization

    IL-2 R alpha Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 26.4 kDa. The protein migrates as 43-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    IL-2 R alpha SDS-PAGE

    Cynomolgus IL-2 R alpha Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IL-2 R alpha SEC-MALS

    The purity of Cynomolgus IL-2 R alpha Protein, His Tag (Cat. No. ILA-C52H8) is more than 90% and the molecular weight of this protein is around 38-53 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     IL-2 R alpha ELISA

    Immobilized Cynomolgus IL-2 R alpha / CD25, His Tag (Cat. No. ILA-C52H8) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-2 Protein, Fc,Avitag™ (Cat. No. IL2-H82F3) with a linear range of 1-20 ng/mL (QC tested).

    Protocol

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バックグランド

Interleukin-2 receptor subunit alpha (IL2RA) is also known as IL-2R subunit alpha, IL-2-RA, IL2-RA, TAC antigen, p55, CD antigen CD25, is a type I transmembrane glycoprotein. IL2RA is expressed on activated T cells and regulatory T cells, and is capable of binding IL2 with low affinity by itself. However, a ligand-induced high affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covelently with the IL2 receptor beta and gamma chain, and subsequently initiates the intacellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been previously regarded as an activation marker, while both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Interleukin-2 receptor alpha chain

  • カテゴリ:

  • 市販の医薬品数:

    3 詳細

  • 治験医薬品数:

    16 詳細

  • 最新の開発ステージ:

    Approved

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