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Product Details
Synonyms
IL2RA, CD25, p55, IL2-RA, IL-2-RA
Source
Mouse IL-2 R alpha, His Tag (ILA-M52H9) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Lys 236 (Accession # P01590-1).
Predicted N-terminus: Glu 22
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.6 kDa. The protein migrates as 40-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Mouse IL-2 R alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Mouse IL-2 R alpha, His Tag (Cat. No. ILA-M52H9) is more than 95% and the molecular weight of this protein is around 30-50 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Human IL-2, Fc Tag, Avi Tag (Cat. No. IL2-H82F3) at 5 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Mouse IL-2 R alpha, His Tag (Cat. No. ILA-M52H9) with a linear range of 5-40 ng/mL (QC tested).
Protocol
Immobilized Mouse IL-2 R alpha, His Tag (Cat. No. ILA-M52H9) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-2, Fc Tag, Avi Tag (Cat. No. IL2-H82F3) with a linear range of 0.6-10 ng/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Mouse IL-2 R alpha, His tag (Cat. No. ILA-M52H9) on HIS1K Biosensor, can bind Human IL-2, Tag Free with an affinity constant of 21.4 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Customer Reviews Writing Reviews
1***22022-08-03
LikesI purchased the company’s mouse-derived CD25 protein for the evaluation of CD25 antibody affinity. It achieved a high sensitivity detection with a small amount! Very satisfied, will come back to purchase more if needed!
Background
Recent Advances
Drug Development Progress- English Name：
Interleukin-2 receptor alpha chain
- Category：
- Approved Drugs：
3 Details
- Drugs in Clinical Trials：
16 Details
- Highest Development Stage：
Approved
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