Product Details
Synonyms
IL-2 R beta & IL-2 R alpha & IL-2 R gamma
Source
Mouse IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag (ILG-M5253) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Glu 240 & Glu 22 - Lys 236 & Trp 23 - Ala 263 (Accession # P16297-1 (IL-2RB) & P01590-1 (IL-2RA) & P34902-1 (IL-2RG)).
Predicted N-terminus: Ala 27 & Glu 22
Request for sequenceMolecular Characterization
Mouse IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag is produced by co-expression of IL-2RB and IL-2RA and IL-2RG, has a calculated MW of 51.5 kDa (IL-2RB) and 79.4 kDa (IL-2RA&IL-2RG). Subunit IL-2RB is fused with a human IgG1 Fc tag at the C-terminus and subunit IL-2RA&IL-2RG is fused with a human IgG1 Fc tag at the C-terminus. The reducing (R) protein migrates as 75-90 kDa and 120-150 kDa respectively due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
After reconstitution, this product is stable after storage at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Mouse IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA

Immobilized Mouse IL-2, His Tag (Cat. No. IL2-M52H3) at 5 μg/mL (100 μL/well) can bind Mouse IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag (Cat. No. ILG-M5253) with a linear range of 0.8-6 ng/mL (QC tested).
Protocol
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Background
Recent Advances
- English Name:
Interleukin-2
- Category:
- Approved Drugs:
2 Details
- Drugs in Clinical Trials:
43 Details
- Highest Development Stage:
Approved
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