Product Details
Synonyms
IL2RB, RP5-1170K4.6, CD122, P70-75
Source
Human IL-2 R beta, Fc Tag (ILB-H5253) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Asp 239 (Accession # P14784-1).
Predicted N-terminus: Ala 27
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 51.0 kDa. The protein migrates as 60-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Human IL-2 R beta, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Human IL-2 R beta, Fc Tag (Cat. No. ILB-H5253) is more than 85% and the molecular weight of this protein is around 110-135 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Human IL-15, premium grade (Cat. No. IL5-H4117) at 5 μg/mL (100 μL/well) can bind Human IL-2 R beta, Fc Tag (Cat. No. ILB-H5253) with a linear range of 5-156 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR

Human IL-2 R beta, Fc Tag (Cat. No. ILB-H5253) captured on CM5 chip via anti-human IgG Fc antibody can bind IL-2, Tag Free with an affinity constant of 0.473 μM as determined in a SPR assay (Biacore T200) (QC tested).
Protocol
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Background
CD122 is also known as IL2R beta, is a member of the type I cytokine receptor family. CD122 is the receptor for interleukin-2 and is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2.
Recent Advances
- English Name:
Interleukin-2 receptor beta chain
- Category:
- Approved Drugs:
0 Details
- Drugs in Clinical Trials:
15 Details
- Highest Development Stage:
Phase 3 Clinical
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