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Product Details
Synonyms
IL2RG, CD132, CIDX, IMD4, P64, SCIDX, SCIDX1, gammaC
Source
Biotinylated Human IL-2 R gamma, His,Avitag (ILG-H85E8) is expressed from Baculovirus-Insect cells. It contains AA Leu 23 - Asn 254 (Accession # P31785-1).
Predicted N-terminus: Leu 23
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 31.0 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human IL-2 R gamma, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA
Immobilized Human IL-2 Protein, Fc Tag (Cat. No. IL2-H5269) at 5 μg/mL (100 μL/well), add 50 μl/well of Biotinylated Human IL-2 R gamma, His,Avitag (Cat. No. ILG-H85E8) at increasing concentrations and then add Human IL-2 R beta, Fc Tag (Cat. No. ILB-H5253) at 5 μg/mL (50 μL/well). Detection was performed using Streptavidin Protein-HRP, Horseradish peroxidase conjugated Streptavidin (Acro, Cat. No. STN-NH913) with sensitivity of 0.06 μg/mL (QC tested).
Protocol
Bioactivity-CELL BASE
Biotinylated Human IL-2 R gamma, His,Avitag (Cat. No. ILG-H85E8) inhibits the IL-2-dependent proliferation of Mo7e cells. The EC50 for this effect is 4.08-5.90 µg/mL in the presence of 10 µg/mL of human IL-2 R beta and 50 ng/mL of human IL-2 (Routinely tested).
Protocol
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Background
Interleukin-2 receptor subunit gamma (IL2RG), also known as cytokine receptor common subunit gamma, CD antigen CD132, gammaC, p64, which belongs to the type I cytokine receptor family or type 5 subfamily. IL2RG is located on the surface of immature blood-forming cells in bone marrow. Defects in IL2RG are the cause of severe combined immunodeficiency X-linked T-cell-negative/B-cell-positive/NK-cell-negative (XSCID).
Recent Advances
Drug Development Progress- English Name：
Interleukin-2 receptor subunit gamma
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
14 Details
- Highest Development Stage：
Phase 2 Clinical
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