Human IL-4 Protein, Fc Tag, low endotoxin (MALS verified)

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製品番号/仕様
価格
数量
IL4-H5253-100ug
$415.00
IL4-H5253-1mg (500ug X 2)
$2855.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    IL4, BCGF1, BSF1

  • Source

    Human IL-4 Protein, Fc Tag (IL4-H5253) is expressed from human 293 cells (HEK293). It contains AA His 25 - Ser 153 (Accession # P05112-1).

    Predicted N-terminus: His 25

    Request for sequence
  • Molecular Characterization

    IL-4 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 41.4 kDa. The protein migrates as 46-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    IL-4 SDS-PAGE

    Human IL-4 Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • SEC-MALS

    IL-4 SEC-MALS

    The purity of Human IL-4 Protein, Fc Tag (Cat. No. IL4-H5253) is more than 95% and the molecular weight of this protein is around 82-110 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     IL-4 ELISA

    Immobilized Human IL-4 R alpha, His Tag (Cat. No. ILR-H5221) at 5 μg/mL (100 μL/well) can bind Human IL-4 Protein, Fc Tag (Cat. No. IL4-H5253) with a linear range of 0.1-1 ng/mL (QC tested).

    Protocol
  •  IL-4 ELISA

    Immobilized Human IL-4 Protein, Fc Tag (Cat. No. IL4-H5253) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-4 R alpha, Avitag,His Tag (Cat. No. ILR-H82E9) with a linear range of 0.1-8 ng/mL (Routinely tested).

    Protocol

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バックグランド

Interleukin-4, is a cytokine that induces differentiation of naive helper T cells (Th0 cells to Th2 cells). In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. Recently, researcher found that the cytokine IL-4 plays a key role in development of innate CD8+ T cells in the thymus of several gene-deficient mouse strains, including Itk, KLF2, CBP and Id3, without previous exposure to antigen.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Interleukin-4

  • カテゴリ:

  • 市販の医薬品数:

    2 詳細

  • 治験医薬品数:

    14 詳細

  • 最新の開発ステージ:

    Approved

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