Biotinylated Human IL-4 R alpha & IL-13 R alpha 1 Protein, His,Avitag™&His Tag (HPLC verified)

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Cat. No. / Size
Price
Qty
ILR-H82W3-25ug
$380.00
ILR-H82W3-200ug
$1565.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    IL-4 R alpha & IL-13 R alpha 1, IL4RA & IL13RA1

  • Source

    Biotinylated Human IL-4 R alpha & IL-13 R alpha 1 Protein, His,Avitag&His Tag (ILR-H82W3) is expressed from human 293 cells (HEK293). It contains AA Met 26 - His 232 & Gly 22 - Thr 343 (Accession # P24394-1 & P78552-1).

    Predicted N-terminus: Met 26 & Gly 22

    Request for sequence
  • Molecular Characterization

    IL-4 R alpha & IL-13 R alpha 1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    Biotinylated Human IL-4 R alpha & IL-13 R alpha 1 Protein, His,Avitag&His Tag is produced by co-expression of IL-4 R alpha and IL-13 R alpha 1, has a calculated MW of 32.1 kDa (IL-4 R alpha) and 43.6 kDa (IL-13 R alpha 1). Subunit IL-4 R alpha is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit IL-13 R alpha 1 is fused with a polyhistidine tag at the C-terminus. The protein migrates as 43-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    IL-4 R alpha & IL-13 R alpha 1 SDS-PAGE

    Biotinylated Human IL-4 R alpha & IL-13 R alpha 1 Protein, His,Avitag&His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-HPLC

    IL-4 R alpha & IL-13 R alpha 1 SEC-HPLC

    The purity of Biotinylated Human IL-4 R alpha & IL-13 R alpha 1 Protein, His,Avitag&His Tag (Cat. No. ILR-H82W3) was greater than 90% as determined by SEC-HPLC.

  • Bioactivity-ELISA

     IL-4 R alpha & IL-13 R alpha 1 ELISA

    Immobilized Biotinylated Human IL-4 R alpha & IL-13 R alpha 1 Protein, His,Avitag&His Tag (Cat. No. ILR-H82W3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human IL-4 Protein, Fc Tag (Cat. No. IL4-H5253) with a linear range of 0.2-5 ng/mL (QC tested).

    Protocol

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Background

Interleukin 4 Receptor (IL4R) also known as CD124, IL4Rα and BSF receptor, is a type I cytokine receptor produced by activated Th2 cells and mast cells, and plays an important role in Th2-­biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis. A soluble form of the encoded IL4R protein can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. IL4R can alternatively associate with IL­13Ra1 to form the type II receptor which is responsive to both IL­4 and IL­13. Interleukin-4 receptor has been shown to interact with SHC1.

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