Cynomolgus Integrin alpha 2 beta 1 (ITGA2&ITGB1) Heterodimer Protein, His Tag&Tag Free (MALS verified)

Synonyms

Integrin alpha 2 beta 1, ITGA2 & ITGB1

Source

Cynomolgus ITGA2&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-C52W5) is expressed from human 293 cells (HEK293). It contains AA Tyr 30 - Pro 1130 (ITGA2) & Gln 161 - Asp 868 (ITGB1) (Accession # G8F2Z5-1 (ITGA2) & A0A7N9D0D7-1 (ITGB1)).

Predicted N-terminus: Tyr 30 (ITGA2) & Gln 161 (ITGB1)

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

Cynomolgus ITGA2&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA2 and ITGB1, has a calculated MW of 127.5 kDa (ITGA2) & 83.6 kDa (ITGB1). Subunit ITGA2 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The protein migrates as 160 kDa (ITGA2) and 110 kDa (ITGB1) when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

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