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Product Details
Synonyms
LAG3, CD223, FDC
Source
Human LAG-3, Fc Tag (LA3-H5255) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Leu 450 (Accession # P18627-1).
Predicted N-terminus: Leu 23
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 72.8 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human LAG-3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human LAG-3, Fc Tag (Cat. No. LA3-H5255) is more than 95% and the molecular weight of this protein is around 160-230 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human LAG-3, Fc Tag (Cat. No. LA3-H5255) at 2 μg/mL (100 μL/well) can bind Madarex LAG-3 MAb with a linear range of 0.4-3 ng/mL (QC tested).
Protocol
Bioactivity-BLI
Loaded Human LAG-3, Fc Tag (Cat. No. LA3-H5255) on Protein A Biosensor, can bind Human FGL1, His Tag (Cat. No. FG1-H52Hy) with an affinity constant of 7.09 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Biotinylated Human FGL1 Protein, His,Avitag (Cat. No. FG1-H82Ey) on SA Biosensor, can bind Human LAG-3 Protein, Fc Tag (Cat. No. LA3-H5255) with an affinity constant of 8.12 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Lymphocyte activation gene 3 protein
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
32 Details
- Highest Development Stage：
Approved
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