Mouse LIGHT / TNFSF14 Protein, His Tag, active trimer (MALS verified)

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製品番号/仕様
価格
数量
LIT-M5243-100ug
$380.00
LIT-M5243-1mg
$2500.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    CD258 antigen, CD258, HVEM-L, LIGHT, LTg, TNFSF14, TR2, HVEML

  • Source

    Mouse LIGHT Protein, His Tag (LIT-M5243) is expressed from human 293 cells (HEK293). It contains AA Asp 72 - Val 239 (Accession # Q9QYH9-1).

    Predicted N-terminus: His

  • Molecular Characterization

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 58.9 kDa. The protein migrates as 55-60 kDa and 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    LIGHT SDS-PAGE

    Mouse LIGHT Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    LIGHT SEC-MALS

    The purity of Mouse LIGHT Protein, His Tag (Cat. No. LIT-M5243) is more than 85% and the molecular weight of this protein is around 60-80 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     LIGHT ELISA

    Immobilized Mouse LIGHT Protein, His Tag (Cat. No. LIT-M5243) at 5 μg/mL (100 μL/well) can bind Human LTBR, Fc Tag (Cat. No. LTR-H5259) with a linear range of 0.1-2 ng/mL (QC tested).

    Protocol
  •  LIGHT ELISA

    Immobilized Mouse LIGHT Protein, His Tag (Cat. No. LIT-M5243) at 5 μg/mL (100 μL/well) can bind Biotinylated Human LTBR, His,Avitag (Cat. No. LTR-H82E9) with a linear range of 4-31 ng/mL (Routinely tested).

    Protocol

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バックグランド

Tumor necrosis factor ligand superfamily member 14(LIGHT) is a tumor necrosis factor (TNF) superfamily ligand that regulates T cell immune responses by signaling through the herpes virus entry mediator (HVEM) and the lymphotoxin beta receptor (LTbetaR). LIGHT has emerged as a potent initiator of T cell co-stimulation signals effecting CTL-mediated tumor rejection, allograft rejection and graft versus host disease. Constitutive expression of LIGHT leads to tissue destruction and autoimmune-like disease syndromes.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Tumor necrosis factor superfamily member 14

  • カテゴリ:

  • 市販の医薬品数:

    0 詳細

  • 治験医薬品数:

    2 詳細

  • 最新の開発ステージ:

    Phase 2 Clinical

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