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製品詳細
Synonyms
CSF1R, C-FMS, CD115, CSFR, FIM2, FMS, M-CSFR
Source
Human M-CSF R, His Tag (CSR-H5228) is expressed from human 293 cells (HEK293). It contains AA Ile 20 - Glu 512 (Accession # NP_005202.2).
Predicted N-terminus: Ile 20
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 55.3 kDa. The protein migrates as 80-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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データ表示
SDS-PAGE
Human M-CSF R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human M-CSF R, His Tag (Cat. No. CSR-H5228) is more than 85% and the molecular weight of this protein is around 70-95 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human M-CSF R, His Tag (Cat. No. CSR-H5228) at 5 μg/mL (100 μL/well) can bind Biotinylated Human M-CSF, His,Avitag (Cat. No. MCF-H82E6) with a linear range of 2-31 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Biotinylated Human M-CSF, His,Avitag (Cat. No. MCF-H82E6) immobilized on SA Chip can bind Human M-CSF R, His Tag (Cat. No. CSR-H5228) with an affinity constant of 0.309 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Human M-CSF, His,Avitag (Cat. No. MCF-H82E6) on SA Biosensor, can bind Human M-CSF R, His Tag (Cat. No. CSR-H5228) with an affinity constant of 378 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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最新の進展
医薬品開発の進捗- 英語名：
Colony stimulating factor 1 receptor
- カテゴリ：
- 市販の医薬品数：
11 詳細
- 治験医薬品数：
38 詳細
- 最新の開発ステージ：
Approved
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