Human MRGPRX2 Full Length Protein, Flag Tag (Nanodisc-pro)

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製品番号/仕様
価格
数量
MR2-H52D3-20ug
$750.00
MR2-H52D3-100ug
$1500.00
MR2-H52D3-500ug
$4800.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Application

    1. Cell based assay (FACS or functional assay)
    2. Affinity testing (SPR / BLI)
    3. Screening (ELISA)
  • Synonyms

    MRGPRX2

  • Source

    Nanodisc-pro Human MRGPRX2 Full Length Protein, Flag Tag (MR2-H52D3) is expressed from human 293 cells (HEK293). It contains AA Asp 2 - Val 330 (Accession # Q96LB1).

    Predicted N-terminus: Asp

    Request for sequence
  • Molecular Characterization

    MRGPRX2 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a flag tag at the N-terminus. The protein has a calculated MW of 53.3 kDa.

    *The isotype control of empty/mock nanodisc (Cat. No. APO-H5143) is sold separately and not included in protein, you can follow the link for product information.

    1. Structure

      Nanodisc-pro membrane protein is wrapped by a piece of cell membrane and scaffold, the info of scaffold can be found in above red text "isotope type control...".

    2. Synthesis Process

      Nanodisc-pro membrane protein extraction is a gentle, non-disruptive process that directly isolates full-length transmembrane proteins together with their surrounding native phospholipids from cellular membranes, without breaking or disturbing the original membrane structure. This preserves the membrane protein's natural conformational landscape and native lipid environment, yielding stable nanodisc that retain cellular phospholipids, with fully preserved biological activity—making them ideal for cell-based assays such as FACS and binding assays in antibody discovery and screening (ELISA), etc.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping and Storage

    This product is supplied and shipped on dry ice.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • Bioactivity-ELISA

     MRGPRX2 ELISA

    Immobilized Human MRGPRX2 Full Length Protein, Flag Tag (Cat. No. MR2-H52D3) at 1 μg/mL (100 μL/well) can bind Anti-MRGPRX2 Antibody, Mouse IgG2b with a linear range of 0.02-1 ng/mL (QC tested).

    Protocol
  • Bioactivity-SPR

     MRGPRX2 SPR

    Human MRGPRX2 Full Length Protein, Flag Tag(Cat. No. MR2-H52D3) immobilized on CM5 Chip can bind Purified anti-human MRGX2 Antibody with an affinity constant of 0.138 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

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バックグランド

The protein belongs to the family of G-protein coupled receptors, that are preferentially activated by adenosine and uridine nucleotides. It is moderately expressed in undifferentiated HL60 cells, and is located on both chromosomes X and Y.

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