Human Neutrophil defensin 1 / DEFA1 protein, Twin-Strep Tag, low endotoxin

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Cat. No. / Size
Price
Qty
ND1-H5282-50ug
$360.00
ND1-H5282-1mg
$3625.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    Neutrophil defensin 1

  • Source

    Human Neutrophil defensin 1, Twin-Strep Tag (ND1-H5282) is expressed from human 293 cells (HEK293). It contains AA Asp 39 - Cys 94 (Accession # P59665-1).

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  • Molecular Characterization

    Neutrophil defensin 1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a twin strep tag at the C-terminus.

    The protein has a calculated MW of 9.8 kDa. The protein migrates as 11-14 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

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  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Neutrophil defensin 1 SDS-PAGE

    Human Neutrophil defensin 1, Twin-Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

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Background

Human defensins are a family of small antimicrobial proteins found predominantly in leukocytes and epithelial cells that play important roles in the innate and adaptive immune defense against microbial infection. The α-defensins, human neutrophil peptides (HNPs) 1–3, are constitutively expressed in neutrophils and are the most abundant neutrophil granule proteins.

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