Biotinylated Mouse PD-L2 / B7-DC Protein, His,Avitag™ (MALS verified)

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製品番号/仕様
価格
数量
PD2-M82E3-25ug
$360.00
PD2-M82E3-200ug
$1420.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    PDL2, PD-L2, Butyrophilin B7-DC, CD273, PDCD1 ligand 2, PDCD1L2, PDCD1LG2

  • Source

    Biotinylated Mouse PD-L2 Protein, His,Avitag (PD2-M82E3) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Arg 219 (Accession # Q9WUL5).

    Predicted N-terminus: Leu 20

    Request for sequence
  • Molecular Characterization

    PD-L2 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 26.1 kDa. The protein migrates as 35-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    PD-L2 SDS-PAGE

    Biotinylated Mouse PD-L2 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    PD-L2 SEC-MALS

    The purity of Biotinylated Mouse PD-L2 Protein, His,Avitag (Cat. No. PD2-M82E3) is more than 95% and the molecular weight of this protein is around 30-40 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     PD-L2 ELISA

    Immobilized Biotinylated Mouse PD-L2 Protein, His,Avitag (Cat. No. PD2-M82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse PD-1 Protein, Mouse IgG2a Fc Tag (Cat. No. PD1-M5257) with a linear range of 1.2-10 ng/mL (QC tested).

    Protocol
  •  PD-L2 ELISA

    Immobilized Human PD-1, Mouse IgG2a Fc Tag (Cat. No. PD1-H5255) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse PD-L2 Protein, His,Avitag (Cat. No. PD2-M82E3) with a linear range of 2-78 ng/mL (Routinely tested).

    Protocol

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バックグランド

Programmed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as Butyrophilin B7-DC, CD antigen CD273, which belongs to the immunoglobulin superfamily or BTN/MOG family. The expression of PD-L2 is up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL-4. PD-L2 Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Programmed death-ligand 2

  • カテゴリ:

  • 市販の医薬品数:

    0 詳細

  • 治験医薬品数:

    5 詳細

  • 最新の開発ステージ:

    Phase 3 Clinical

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