Human Prostatic Acid Phosphatase Protein, His Tag, low endotoxin (active enzyme, MALS verified)

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製品番号/仕様
価格
数量
PRE-H52H3-20ug
$230.00
PRE-H52H3-100ug
$890.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    Prostatic Acid Phosphatase, ACPP, ACP-3, PAP, 5'-NT, TMPase, TM-PAP

  • Source

    Human Prostatic Acid Phosphatase Protein, His Tag (PRE-H52H3) is expressed from human 293 cells (HEK293). It contains AA Lys 33 - Lys 382 (Accession # P15309-2).

    Predicted N-terminus: Lys 33

    Request for sequence
  • Molecular Characterization

    Prostatic Acid Phosphatase Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 42.5 kDa. The protein migrates as 45-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

    The protein is designed as a dimer.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Supplied as 0.2 μm filtered solution in 100 mM NaAC, pH5.0 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    Prostatic Acid Phosphatase SDS-PAGE

    Human Prostatic Acid Phosphatase Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Prostatic Acid Phosphatase SEC-MALS

    The purity of Human Prostatic Acid Phosphatase Protein, His Tag (Cat. No. PRE-H52H3) is more than 95% and the molecular weight of this protein is around 85-105 kDa verified by SEC-MALS.

    Report
  • Bioactivity

    Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP). The specific activity is >95,000 pmol/min/ug (QC tested).

    Protocol

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バックグランド

Prostatic acid phosphatase also know as acid phosphatase 3 (ACP3), it is a non-specific tyrosine phosphatase that acts as a tumor suppressor of prostate cancer through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling. In addition to its tyrosine phosphatase activity has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP which acts as a pain suppressor.

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