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Product Details
Synonyms
CD265, FEO, LOH18CR1, ODFR, OFE, OPTB7, OSTS, PDB2, RANK, TNFRSF11A, TRANCER, CD265 antigen, ODFROSTS, RANKLOH18CR1, NFKB activator
Source
Human RANK Protein, Mouse IgG2a Fc Tag (RAK-H5251) is expressed from human 293 cells (HEK293). It contains AA Ile 30 - Pro 212 (Accession # Q9Y6Q6-1).
Predicted N-terminus: Ile 30
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 47.2 kDa. The protein migrates as 50-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human RANK Protein, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human RANK Protein, Mouse IgG2a Fc Tag (Cat. No. RAK-H5251) is more than 90% and the molecular weight of this protein is around 95-120 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human TNFSF11, His Tag (Cat. No. RAL-H5240) at 2 μg/mL (100 μL/well) can bind Human RANK Protein, Mouse IgG2a Fc Tag (Cat. No. RAK-H5251) with a linear range of 0.4-13 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Captured Human RANK Protein, Mouse IgG2a Fc Tag (Cat. No. RAK-H5251) on CM5 chip via Anti-Mouse antibodies surface can bind Human TNFSF11, His Tag (Cat. No. RAL-H5240) with an affinity constant of 0.309 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human RANK Protein, Mouse IgG2a Fc Tag (Cat. No. RAK-H5251) on AMC Biosensor, can bind Human TNFSF11 Protein, His Tag (Cat. No. RAL-H5240) with an affinity constant of 0.409 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Customer Reviews Writing Reviews
1***22019-10-10
LikesThere was only mouse IgG for TNFRSF11A for now. I tried the SDS-PAGE, and the gray scale came out fine. The molecular data was similar to the one on their official website.
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