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製品詳細
Synonyms
ROR1, NTRKR1
Source
Human / Cynomolgus / Rhesus macaque ROR1, His Tag (RO1-H522y) is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Glu 403 (Accession # Q01973-1). In the region Gln 30 - Glu 403, the amino acid sequences of Human, Cynomolgus and Rhesus macaque ROR1 are identical.
Predicted N-terminus: Gln 30
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 43.9 kDa. The protein migrates as 55-66 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 30 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
ACRO Quality Management System
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データ表示
SDS-PAGE
Human / Cynomolgus / Rhesus macaque ROR1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human / Cynomolgus / Rhesus macaque ROR1, His Tag (Cat. No. RO1-H522y) is more than 95% and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human / Cynomolgus / Rhesus macaque ROR1, His Tag (Cat. No. RO1-H522y) at 2 μg/mL (100 μL/well) can bind Anti-ROR1 Antibody, Human IgG1 with a linear range of 0.2-2 ng/mL (QC tested).
Protocol
Immobilized Human / Cynomolgus / Rhesus macaque ROR1, His Tag (Cat. No. RO1-H522y) at 1 μg/mL (100 μL/well) can bind Zilovertamab with a linear range of 0.06-1 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Anti-Human ROR1 mAb captured on CM5 chip via anti-mouse antibodies surface can bind Human / Cynomolgus / Rhesus macaque ROR1, His Tag (Cat. No. RO1-H522y) with an affinity constant of 4.9 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Zilovertamab captured on Protein A Chip can bind Human / Cynomolgus / Rhesus macaque ROR1, His Tag (Cat. No. RO1-H522y) with an affinity constant of 83.1 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Anti-Human ROR1 MAb (Mouse IgG1) on AMC Biosensor, can bind Human / Cynomolgus / Rhesus macaque ROR1, His Tag (Cat. No. RO1-H522y) with an affinity constant of 10.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Zilovertamab on Protein A Biosensor, can bind Human / Cynomolgus / Rhesus macaque ROR1, His Tag (Cat. No. RO1-H522y) with an affinity constant of 38.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Bioactivity-FACS
2e5 of anti-ROR1 CAR-293 cells were stained with 100 μL of 10 μg/mL of Human / Cynomolgus / Rhesus macaque ROR1, His Tag (Cat. No. RO1-H522y) and negative control protein respectively, washed and then followed by PE-anti-His Tag antibody and analyzed with FACS (Routinely tested).
Protocol
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最新の進展
医薬品開発の進捗- 英語名：
Inactive tyrosine-protein kinase transmembrane receptor ROR1
- カテゴリ：
- 市販の医薬品数：
0 詳細
- 治験医薬品数：
22 詳細
- 最新の開発ステージ：
Phase 3 Clinical
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