Human Siglec-6 / CD327 Protein, Fc Tag, low endotoxin

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製品番号/仕様
価格
数量
SI6-H5256-100ug
$310.00
SI6-H5256-1mg (250ug X 4)
$1905.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    SIGLEC6, CD327, CD33L, CD33L1, OBBP1, OB-BP1, CDw327

  • Source

    Human Siglec-6, Fc Tag (SI6-H5256) is expressed from human 293 cells (HEK293). It contains AA Gln 16 - Val 320 (Accession # AAH35359).

    Predicted N-terminus: Gln 16

    Request for sequence
  • Molecular Characterization

    Siglec-6 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 60.3 kDa. The protein migrates as 80-100 kDa under reducing (R) condition, and 130-150 kDa and 250-300 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    Siglec-6 SDS-PAGE

    Human Siglec-6, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     Siglec-6 ELISA

    Immobilized Human Siglec-6, Fc Tag (Cat. No. SI6-H5256) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human Siglec-6 Antibody, Rabbit IgG with a linear range of 0.2-16 ng/mL (Routinely tested).

    Protocol
  •  Siglec-6 ELISA

    Immobilized Human Siglec-6, Fc Tag (Cat. No. SI6-H5256) at 1 μg/mL (100 μL/well) can bind Human siglec-6/D327 Antibody, Sheep IgG with a linear range of 0.2-8 ng/mL (Routinely tested).

    Protocol

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コメントを投稿

  • C***u2022-06-25
    いいね
    The item was expedited very quickly which worked out with our timeline. We are in the process of using the Siglec-6 protein in one of our in-vivo study for immunization. The material itself did not dissolve in complete freund's adjuvant but it was easily made into a fine suspension with the help of some PBS. Overall, I would say the material is easy to work with and the Acro is quick at responding if we have any issues.

バックグランド

Sialic acid-binding Ig-like lectin 6 (SIGLEC6) is also known as CD antigen CD327, CD33 antigen-like 1 (CD33L or CD33L1), Obesity-binding protein 1 (OB-BP1). SIGLEC6 belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family, which contains two Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. SIGLEC6 mediates sialic-acid dependent binding to cells. SIGLEC6 binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Sialic acid-binding Ig-like lectin 6

  • カテゴリ:

  • 市販の医薬品数:

    0 詳細

  • 治験医薬品数:

    2 詳細

  • 最新の開発ステージ:

    Phase 2 Clinical

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