Human STAT3 Protein, His Tag, low endotoxin (MALS & SPR verified)

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製品番号/仕様
価格
数量
ST3-H5149-100ug
$410.00
ST3-H5149-500ug (250ug X 2)
$1230.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    STAT3, signal transducer and activator of transcription 3

  • Source

    Human STAT3, His Tag (ST3-H5149) is expressed from E. coli cells. It contains AA Gly 127 - Arg 688 (Accession # P40763-1).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    STAT3 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 66.4 kDa. The protein migrates as 66-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    STAT3 SDS-PAGE

    Human STAT3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    STAT3 SEC-MALS

    The purity of Human STAT3, His Tag (Cat. No. ST3-H5149) is more than 85% and the molecular weight of this protein is around 60-75 kDa verified by SEC-MALS.

    Report
  • Bioactivity-SPR

     STAT3 SPR

    Human STAT3, His Tag (Cat. No. ST3-H5149) immobilized on CM5 Chip can bind STAT3 degrader with an affinity constant of 44.6 nM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol
  • Bioactivity-BLI

     STAT3 BLI

    Loaded Human STAT3, His Tag (Cat. No. ST3-H5149) on NTA Biosensor, can bind STAT3 degrader with an affinity constant of 29.4 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

    Protocol

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バックグランド

Signal transducer and activator of transcription 3 (STAT3) regulates the expression of genes important for essential cellular processes including survival, proliferation, self-renewal, angiogenesis, and immune response. As an early tumor diagnostic marker, it is considered that constitutive STAT3 activation is a sufficient factor to cause malignant transformation and tumor development in model systems. The classical IL-6/STAT3 signaling pathway in cancer cells. IL-6 binds to the membrane-bound IL-6 receptor α (IL-6R) and IL-6 receptor β (also known as gp130). The IL-6/IL-6R/gp130 complex activate the phosphorylation of JAKs, followed by STAT3 phosphorylation and activation. Then, phosphorylated STAT3 forms a homodimer and translocates into the nucleus to bind to the promotor region of target genes and activates target gene transcription.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Signal transducer and activator of transcription 3

  • カテゴリ:

  • 市販の医薬品数:

    4 詳細

  • 治験医薬品数:

    21 詳細

  • 最新の開発ステージ:

    Approved

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