Mouse Thrombopoietin R Protein, His Tag, low endotoxin (MALS & SPR verified)

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製品番号/仕様
価格
数量
THR-M52H3-100ug
$395.00
THR-M52H3-1mg (200ug X 5)
$2620.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    MPL, C-MPL, CD110, MPLV, THCYT2, TPOR

  • Source

    Mouse Thrombopoietin R Protein, His Tag (THR-M52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Trp 482 (Accession # Q08351-1).

    Predicted N-terminus: Gln 26

  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 53.1 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    Thrombopoietin R SDS-PAGE

    Mouse Thrombopoietin R Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Thrombopoietin R SEC-MALS

    The purity of Mouse Thrombopoietin R Protein, His Tag (Cat. No. THR-M52H3) is more than 95% and the molecular weight of this protein is around 50-70 kDa verified by SEC-MALS.

    Report
  • Bioactivity-SPR

     Thrombopoietin R SPR

    Mouse Thrombopoietin R Protein, His Tag (Cat. No. THR-M52H3) capture on NTA-Series S sensor chip can bind Human Thrombopoietin Protein, Tag Free with an affinity constant of 5.67 nM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol

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バックグランド

Thrombopoietin R, also known as TPO-R, is expressed predominantly on the surface of MKs, platelets, hemangioblasts, and hematopoietic stem cells (HSCs). Binding of TPO to the megakaryocyte TPO-R leads to different effects: prevention of megakaryocyte apoptosis; increased megakaryocyte number, size, and ploidy; increasing rate of megakaryocyte maturation; and internalization of the TPO/TPO-R complex. Thrombopoietin R involved in multiple signal transduction pathways, such as JAK, STAT, and MAP kinase.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Thrombopoietin receptor

  • カテゴリ:

  • 市販の医薬品数:

    8 詳細

  • 治験医薬品数:

    11 詳細

  • 最新の開発ステージ:

    Approved

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