Human TNFR1 / CD120a / TNFRSF1A Protein, His Tag, low endotoxin (MALS verified)

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製品番号/仕様
価格
数量
TN1-H5222-100ug
$310.00
TN1-H5222-1mg (500ug X 2)
$2280.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    TNFR1, CD120a, TNFRSF1A, FPF, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR55, TNFR60, p55, p60

  • Source

    Human TNFR1, His Tag (TN1-H5222) is expressed from human 293 cells (HEK293). It contains AA Leu 30 - Thr 211 (Accession # P19438).

    Predicted N-terminus: Leu 30

    Request for sequence
  • Molecular Characterization

    TNFR1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 21.6 kDa. The protein migrates as 28-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to different glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    TNFR1 SDS-PAGE

    Human TNFR1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    TNFR1 SEC-MALS

    The purity of Human TNFR1, His Tag (Cat. No. TN1-H5222) is more than 95% and the molecular weight of this protein is around 25-35 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     TNFR1 ELISA

    Immobilized Human TNF-alpha, premium grade (Cat. No. TNA-H4211) at 2 μg/mL (100 μL/well) can bind Human TNFR1, His Tag (Cat. No. TN1-H5222) with a linear range of 3-25 ng/mL (QC tested).

    Protocol

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バックグランド

Tumor necrosis factor receptor 1 (TNF-R1) is also known as Tumor necrosis factor receptor superfamily member 1A (TNFRSF1A), TNFAR, CD antigen CD120a, which belongs to the tumor necrosis factor receptor superfamily. TNF-R1 contains one death domain and four TNFR-Cys repeats. TNF-R1 is the receptor of TNFSF2 / TNF-alpha and homotrimeric TNFSF1 / lymphotoxin - alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. TNF-R1 contributes to the induction of non - cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Defects in TNFRSF1A are the cause of familial hibernian fever (FHF).

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Tumor necrosis factor receptor superfamily member 1A

  • カテゴリ:

  • 市販の医薬品数:

    0 詳細

  • 治験医薬品数:

    2 詳細

  • 最新の開発ステージ:

    Phase 3 Clinical

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