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製品詳細
Synonyms
RANKL, CD254, TRANCE, OPGL, ODF
Source
Cynomolgus TNFSF11 Protein, His Tag (RAL-C5243) is expressed from human 293 cells (HEK293). It contains AA Gly 136 - Asp 317 (Accession # A0A7N9DBU4).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 22.4 kDa. The protein migrates as 30-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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データ表示
SDS-PAGE
Cynomolgus TNFSF11 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Cynomolgus TNFSF11 Protein, His Tag (Cat. No. RAL-C5243) is more than 85% and the molecular weight of this protein is around 75-85 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human RANK Protein, Mouse IgG2a Fc Tag (Cat. No. RAK-H5251) at 1 μg/mL (100 μL/well) can bind Cynomolgus TNFSF11 Protein, His Tag (Cat. No. RAL-C5243) with a linear range of 0.5-15 ng/mL (QC tested).
Protocol
Immobilized Cynomolgus TNFSF11 Protein, His Tag (Cat. No. RAL-C5243) at 1 μg/mL (100 μL/well) can bind Human RANK Protein, Mouse IgG2a Fc Tag (Cat. No. RAK-H5251) with a linear range of 0.03-1 ng/mL (Routinely tested).
Protocol
Immobilized Cynomolgus TNFSF11 Protein, His Tag (Cat. No. RAL-C5243) at 1 μg/mL (100 μL/well) can bind Anti-TNFSF11 Antibody, Human IgG1 with a linear range of 0.06-1 ng/mL (Routinely tested).
Protocol
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最新の進展
医薬品開発の進捗- 英語名：
Tumor necrosis factor ligand superfamily member 11
- カテゴリ：
- 市販の医薬品数：
18 詳細
- 治験医薬品数：
18 詳細
- 最新の開発ステージ：
Approved
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