Product Details
Synonyms
KDR, CD309, FLK1, VEGFR, VEGFR2
Source
Human VEGF R2, Fc Tag (VE2-H5255) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Glu 764 (Accession # AAI31823.1).
Predicted N-terminus: Ala 20
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 109.7 kDa. The protein migrates as 140-170 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Human VEGF R2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS

The purity of Human VEGF R2, Fc Tag (Cat. No. VE2-H5255) is more than 95% and the molecular weight of this protein is around 256-313 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Human VEGF165, Tag Free at 2 μg/mL (100 μL/well) can bind Human VEGF R2, Fc Tag (Cat. No. VE2-H5255) with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
Immobilized Human VEGF R2, Fc Tag (Cat. No. VE2-H5255) at 1 μg/mL (100 μL/well) can bind Anti-VEGFR2 Antibody, Mouse IgG1 with a linear range of 0.06-2 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
- English Name:
Vascular endothelial growth factor receptor 2
- Category:
- Approved Drugs:
18 Details
- Drugs in Clinical Trials:
61 Details
- Highest Development Stage:
Approved
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