Biotinylated Mouse VEGF R2 / KDR Protein, His,Avitag™ (MALS verified)

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製品番号/仕様
価格
数量
VE2-M82E3-25ug
$360.00
VE2-M82E3-200ug
$1565.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    KDR, CD309, FLK1, VEGFR, VEGFR2

  • Source

    Biotinylated Mouse VEGF R2 Protein, His,Avitag (VE2-M82E3) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Glu 762 (Accession # P35918-1).

    Predicted N-terminus: Ala 20

    Request for sequence
  • Molecular Characterization

    VEGF R2 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 86.6 kDa. The protein migrates as 105-135 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    VEGF R2 SDS-PAGE

    Biotinylated Mouse VEGF R2 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    VEGF R2 SEC-MALS

    The purity of Biotinylated Mouse VEGF R2 Protein, His,Avitag (Cat. No. VE2-M82E3) is more than 95% and the molecular weight of this protein is around 100-130 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     VEGF R2 ELISA

    Immobilized Biotinylated Mouse VEGF R2 Protein, His,Avitag (Cat. No. VE2-M82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse VEGF-D Protein, Fc Tag (Cat. No. VED-M5253) with a linear range of 0.6-39 ng/mL (QC tested).

    Protocol

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バックグランド

Kinase insert domain receptor (KDR) is also known as CD309, FLK1, VEGFR, VEGFR2, and is one of the subtypes of VEGFR. VEGF receptors are receptors for vascular endothelial growth factor (VEGF). There are three main subtypes of VEGFR, numbered 1, 2 and 3. The VEGF receptors have an extracellular portion consisting of 7 immunoglobulin-like domains, a single transmembrane spanning region and an intracellular portion containing a split tyrosine-kinase domain. VEGF-A binds to VEGFR-1 (Flt-1) and VEGFR-2 (KDR/Flk-1). VEGFR-2 appears to mediate almost all of the known cellular responses to VEGF.The function of VEGFR-1 is less well defined, although it is thought to modulate VEGFR-2 signaling. Another function of VEGFR-1 may be to act as a dummy/decoy receptor, sequestering VEGF from VEGFR-2 binding (this appears to be particularly important during vasculogenesis in the embryo). In addition, VEGFR2 is able to interact with HIV-1 extracellular Tat protein upon VEGF activation, and seems to enhance angiogenesis in Kaposi's sarcoma lesions.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Vascular endothelial growth factor receptor 2

  • カテゴリ:

  • 市販の医薬品数:

    18 詳細

  • 治験医薬品数:

    61 詳細

  • 最新の開発ステージ:

    Approved

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