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Product Details
Synonyms
RP1-261G23.1, MGC70609, MVCD1, VEGFA, VPF
Source
Human VEGF110 Protein, premium grade (VE0-H5212) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 136 (Accession # NP_001165097).
Predicted N-terminus: Ala 27
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries no "tag".
The protein has a calculated MW of 12.7 kDa. The protein migrates as 16-18 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.2 EU per μg by the LAL method / rFC method.
Sterility
Negative
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human VEGF110 Protein, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human VEGF110 Protein, premium grade (Cat. No. VE0-H5212) is more than 85% and the molecular weight of this protein is around 28-38 kDa verified by SEC-MALS.
Report
Bioactivity-Organoid Culture
iPSC derived blood vessel organoids forming net-works were cultured with VEGF110 (Cat. No. VE0-H5212).
iPSC derived liver organoids forming cystic structure of bile duct were cultured with VEGF110 (Cat. No. VE0-H5212).
Bioactivity-CELL BASE
Human VEGF110 Protein, premium grade (Cat. No. VE0-H5212) stimulates proliferation of human umbilical vein endothelial cells (HUVEC). The EC50 for this effect is 5.06-11.59 ng/mL (Routinely tested).
Protocol
Bioactivity-ELISA
Immobilized Human VEGF110 Protein, premium grade (Cat. No. VE0-H5212) at 0.05 μg/mL (100 μL/well) can bind Human VEGF R1 Protein, His Tag (Cat. No. VE1-H52H9) with a linear range of 2-16 ng/mL (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Vascular endothelial growth factor A
- Category：
- Approved Drugs：
54 Details
- Drugs in Clinical Trials：
61 Details
- Highest Development Stage：
Approved
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