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Product Details
Synonyms
RP1-261G23.1, MGC70609, MVCD1, VEGFA, VPF
Source
Mouse VEGF164 Protein, His Tag (VE4-M5243) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 190 (Accession # Q00731-2).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 21.2 kDa. The protein migrates as 26-31 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Mouse VEGF164 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Mouse VEGF164 Protein, His Tag (Cat. No. VE4-M5243) is more than 95% and the molecular weight of this protein is around 45-60 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Mouse VEGF R1 Protein, Mouse IgG2a Fc Tag (Cat. No. VE1-M5256) at 2 μg/mL (100 μL/well) can bind Mouse VEGF164 Protein, His Tag (Cat. No. VE4-M5243) with a linear range of 0.5-63 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Mouse VEGF R2 Protein, Mouse IgG2a Fc Tag (Cat. No. VE2-M5258) captured on CM5 chip via anti-mouse antibodies surface can bind Mouse VEGF164 Protein, His Tag (Cat. No. VE4-M5243) with an affinity constant of 0.032 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Vascular endothelial growth factor A
- Category：
- Approved Drugs：
55 Details
- Drugs in Clinical Trials：
56 Details
- Highest Development Stage：
Approved
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