Human VEGF165 Protein, His Tag, low endotoxin (MALS verified)

SDS-PAGE

Human VEGF165, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS

The purity of Human VEGF165, His Tag (Cat. No. VE5-H5248) is more than 95% and the molecular weight of this protein is around 42-60 kDa verified by SEC-MALS.

Bioactivity-ELISA

Immobilized Human VEGF R1&VEGF R2, Fc Tag at 2 μg/mL (100 μL/well) can bind Human VEGF165, His Tag (Cat. No. VE5-H5248) with a linear range of 0.004-0.063 μg/mL (QC tested).

Bioactivity-TR-FRET

In this TR-FRET assay, Streptavidin Protein Europium chelate (for TR-FRET) (Cat. No. FRT-TD09) is used as the Donor and Monoclonal Anti-His Tag Antibody, Rabbit IgG FA-Labeled (for TR-FRET) (Cat. No. FRT-TA11) is used as the Acceptor. Human VEGF165, His Tag (Cat. No. VE5-H5248), at 1.25 ng/well and Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1), at 1.25 ng/well, can bind to the Ivonescimab with a linear detection range of 2-0.002 μg/mL (Routinely tested).

Bridging ELISA

Validated with Bispecific Antibody.
Immobilized Human VEGF165 Protein, His Tag, low endotoxin (MALS verified) (Cat. No. VE5-H5248) at 1 μg/mL, add increasing concentrations of Ivonescimab and then add Biotinylated Cynomolgus PD-1 / PDCD1 Protein, Fc,Avitag™ (MALS verified) (Cat. No. PD1-C82F7) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 0.244 ng/mL (Routinely tested).

Validated with Bispecific Antibody.
Immobilized Human VEGF165, His Tag (Cat. No. VE5-H5248) at 1 μg/mL, add increasing concentrations of Pumitamig and then add Biotinylated Human PD-L1, Avitag,His Tag (Cat. No. PD1-H82E5) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 0.31 ng/mL (Routinely tested).

Bioactivity-SPR

Avastin (Bevacizumab) captured on CM5 chip via ant-human IgG Fc antibodies surface, can bind Human VEGF165 Protein, His Tag (Cat. No. VE5-H5248) with an affinity constant of 1.03 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI

Loaded Human NRP1, Fc Tag (Cat. No. NR1-H5252) on Protein A Biosensor, can bind Human VEGF165, His Tag (Cat. No. VE5-H5248) with an affinity constant of 24.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Loaded Biotinylated Human VEGF R1, His,Avitag (Cat. No. VE1-H82E3) on SA Biosensor, can bind Human VEGF165, His Tag (Cat. No. VE5-H5248) with an affinity constant of 0.236 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).