Coxsackievirus A16 (strain G-10) VP0 Protein, His Tag

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Cat. No. / Size
Price
Qty
VP0-C52H3-50ug
$465.00
VP0-C52H3-500ug
$2370.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    VP0 (Coxsackievirus A16)

  • Source

    Coxsackievirus A16 (strain G-10) VP0 Protein, His Tag (VP0-C52H3) is expressed from human 293 cells (HEK293). It contains AA Gly 2 - Gln 323 (Accession # Q65900-1).

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  • Molecular Characterization

    VP0 (Coxsackievirus A16) Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 37.6 kDa. The protein migrates as 40-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

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  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    VP0 (Coxsackievirus A16) SDS-PAGE

    Coxsackievirus A16 (strain G-10) VP0 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     VP0 (Coxsackievirus A16) ELISA

    Immobilized Coxsackievirus A16 (strain G-10) VP0 Protein, His Tag (Cat. No. VP0-C52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Coxsackievirus A16 (strain G-10) VP0 Antibody, Human IgG1 (14E1) (Cat. No. VP0-MY2108) with a linear range of 0.1-4 ng/mL (Routinely tested).

    Protocol

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Background

Cox A16 infection is very common in China, and the pathogenicity of Cox A16 is significantly different from enterovirus 71. Cox A16 can induce the production of a variety of micribonucleic acid, which affects the conduction of inflammatory signaling pathway, leading to the occurrence of pathological damage. Cox A16 capsid protein VP1, as an important target protein, interacts with host cell receptors to provide virion attachment to target host cells.

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