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Biotinylated Cynomolgus Mafa-A1*063:01&B2M&RM9 (RPKVPLRTM) Complex Protein (Monomer, MALS verified)

  • Source
    Biotinylated Cynomolgus Mafa-A1*063:01&B2M&RM9 (RPKVPLRTM) Complex Protein(MA9-C82T6) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Pro 307 (Mafa-A1*063:01) & Ile 21 - Met 119 (B2M) & RPKVPLRTM peptide (Accession # AAY57215.2 (Mafa-A1*063:01) & Q8SPW0 (B2M) & RPKVPLRTM).
    Predicted N-terminus: Gly 25 & Arg
  • Molecular Characterization

    Biotinylated Cynomolgus Mafa-A1*063:01&B2M&RM9 (RPKVPLRTM) Complex Protein is produced by co-expression of Mafa-A1 and B2M loaded with RM9 peptide.

    This protein carries a flag tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 35.8 kDa and 13.8 kDa. The protein migrates as 40-43 kDa and 12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mafa-A1*06301 & B2M & RM9 (RPKVPLRTM) SDS-PAGE

Biotinylated Cynomolgus Mafa-A1*063:01&B2M&RM9 (RPKVPLRTM) Complex Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Mafa-A1*06301 & B2M & RM9 (RPKVPLRTM) MALS images

The purity of Biotinylated Cynomolgus Mafa-A1*063:01&B2M&RM9 (RPKVPLRTM) Complex Protein (Cat. No. MA9-C82T6) is more than 90% and the molecular weight of this protein is around 45-65 kDa verified by SEC-MALS.

  • Clinical and Translational Updates

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