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Your Position: ホーム > High Quality Laminin Protein Supports Clinical iPSC Banking

High Quality Laminin Protein Supports
Clinical iPSC Banking

Pre-coating and Suspension Methods

Laminin 521 Laminin 511

High Quality Laminin Protein supports Clinical iPSC Banking

Induced pluripotent stem cells (iPSCs) are becoming increasingly vital in developing relevant cell models and advancing regenerative medicine. Despite their potential, the considerable time and expense required for iPSC generation present ongoing challenges for researchers. The rise of iPSC banks offers a promising solution by improving access to these valuable cells and standardizing their quality and reliability. However, establishing effective iPSC banks demands overcoming critical challenges, including achieving rapid cell expansion, maintaining stemness and differentiation potential, and ensuring genomic and karyotypic stability. High-quality iPSC banks are essential for addressing these challenges and driving further research progress. In this context, our recombinant Laminin proteins offer an superior solution for iPSC banking.

High Quality Laminin Protein supports Clinical iPSC Banking

RCB: Research Cell Bank        PCB: Primary Cell Bank
WCB: Working Cell Bank        MCB: Master Cell Bank

Innovative Laminin-Based iPSC Culture Methods

At the forefront of iPSC expansion and culture research, we offer innovative solutions for your iPSC banking needs. Our high-quality products, Laminin 521 and Laminin 511, are versatile and adaptable to a wide range of culture requirements. Laminin 521 features advanced pre-coating technology, while Laminin 511 offers a convenient Suspension Methods approach. Both options are engineered to support efficient large-scale iPSC cultivation, ensuring optimal results for your research needs. Whether you're focused on basic science or clinical applications, we have the ideal Laminin protein to match your specific needs and elevate your iPSC research to new heights.

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Pre-coating Method

Pre-coating plates with Laminin 521 creates an ideal environment for low-density single-cell seeding, enabling cells to exhibit high motility and achieve excellent clonal survival. This process promotes the formation of a tightly arranged monolayer, ensuring efficient long-term culture of human pluripotent stem cells. Laminin 521 offers a significant advantage over feeder cell-dependent methods by delivering consistent batch-to-batch performance and providing a well-defined chemical composition. By eliminating the risk of external contamination, and enhancing overall culture efficiency, Laminin 521 empowers researchers to conduct more reliable and reproducible stem cell studies.

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Suspension Methods

Our Laminin 511 features an innovative Suspension Methods,  eliminating the need for plate coating prior to cell seeding. This approach addresses common concerns about lengthy curing times or plates drying out, while significantly reducing material usage. By requiring only half the typical pre-coating material, Laminin 511 enables more cost-effective and resource-efficient research. Its compatibility with automated systems simplifies workflows, reducing steps and enhancing overall efficiency. Laminin 511 is the ideal choice for researchers seeking to optimize their stem cell culture processes, saving time and resources without compromising quality.

Excellent Performance——Laminin 521 & Laminin 511

GMP-grade Laminin 521 Supports iPSC Expansion with Pre-coating Method

iPSC-T
Accelerated Performance - Drive rapid expansion of hPSC and ensure seamless differentiation into a wide variety of specialized cell types.
Enhanced Adhesion -  Achieve superior adhesion even at a concentration as low as 2μg/ml.
Stemness Maintenance -  Preserve stem cell integrity with no spontaneous differentiation across multiple passages, maintaining your research precison.
Unmatched Consistency - Benefit from reliable lot-to-lot consistency, thanks to our stable cell line production, robust purification process, and stringent QC.
Scale-up Ready - Secure a seamless transition to large-scale production with our cGMP-compliant facility.
High Quality Laminin Protein supports Clinical iPSC Banking

Laminin 521 (GMP-LA5H24) effectively supports the expansion of hPSC.

High Quality Laminin Protein supports Clinical iPSC Banking

Laminin 521 (GMP-LA5H24) maintains the stemness of iPSC across multiple passages

High Quality Laminin Protein supports Clinical iPSC Banking

Normal karyotype (46, XX) was observed in hPSCs with Laminin 521(GMP-LA5H24) coating after 10 passages.

Premium-grade Laminin 511 Supports iPSC Expansion with Suspension Method

iPSC-T
Automation-Ready - Simplify your workflow with easy-to-use, no coating required method, perfect for automated systems.
Cost Effective -  Cut costs by half of amount (0.25μg/cm2) required in traditional coating methods, maximizing your budget.
Stemness Maintenance -  Maintain the purity of your hPSCs with no spontaneous differentiation observed over multiple passages, ensuring reliable results.
Unmatched Consistency - Experience reliable lot-to-lot consistency, backed by production from a stable cell line, rigorous purification ,and stringent QC.
Scale-up Ready - Secure a seamless transition to large-scale production with our cGMP-compliant facility.
High Quality Laminin Protein supports Clinical iPSC Banking

Robust, and homogeneous, monolayer expansion of single cell hPSCs on Laminin 511 (LA1-H5213)

High Quality Laminin Protein supports Clinical iPSC Banking
High Quality Laminin Protein supports Clinical iPSC Banking

hPSCs cultured on Laminin 511 (LA1-H5213) maintain pluripotency, demonstrating high expression of OCT4, SOX2, and SSEA4 as confirmed by FACS and immunofluorescent staining.

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Related Products list

Matrix ProteinsCatalog No.ProductApplication
Laminin 521GMP-LA5H24GMP Human Laminin 521 ProteinHuman PSC expansion in the clinical stage
Laminin 521LA5-H5261Human Laminin 521 Protein, premium gradeHuman PSC expansion in the pre-clinical stage
Laminin 511Coming soon!GMP Human Laminin 511 Protein (EasyScale)Human PSC expansion in the clinical stage
Laminin 511LA1-H5213Human Laminin 511 Protein, premium grade (EasyScale)Human PSC expansion in the pre-clinical stage
VitronectinVIN-H5117Human Truncated Vitronectin (VTN-N) Protein, premium gradeSupports human PSC expansion in the pre-clinical stage
FibronectinFIN-H5113Recombinant Fibronectin fragment, premium gradeMesenchymal stem cell attachment and proliferation
Laminin 111LA1-H5269Human Laminin 111 Protein, premium gradeHepatic and neural differentiation
Laminin 121LA1-H5261Human Laminin 121 Protein, premium gradeEspecially hepatic and neural cells among many other cell types
Laminin 211LA8-H5263Human Laminin 211 Protein, premium gradeSkeletal muscle, neural and cardiac cells differentiation
Laminin 221LA2-H5269Human Laminin 221 Protein, premium gradeCardiac cells differentiation
Laminin 411LAT-H5263Human Laminin 411 Protein, premium gradeEndothelial and pancreatic cells differentiation

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Frequently Asked Questions

How do I choose the appropriate grades of Laminin 521?  

For pre-clinical translational researchers, we recommend our premium-grade Laminin 521, a reliable and cost-effective option for stem cell research. Compared to other commercial extracellular matrices, it offers superior performance and value. For clinical applications, the GMP-grade Laminin 521 is designed to support large-scale iPSC manufacturing, produced under rigorous GMP standards to ensure compliance with global regulatory requirements.

What are the advantages of recombinant Laminin 521 compared to other commercial basement membrane extracts?

Recombinant Laminin 521 offers a defined cell matrix with superior lot-to-lot consistency. It supports faster stem cell growth in homogenous monolayer compared to basement membrane extracts. In addition, it is widely used for in vitro feeder-free culture of various hPSCs while maintaining high quality.

How can I transfer my stem cells from other commercial extracellular matrix proteins, such as Matrigel, Laminin to your Laminin 521?

Transferring your stem cells to Laminin 521 is straightforward, requiring no special adaptation process. Whether you’re transitioning from Matrigel, Vitronectin and other Laminin protein, we recommend adapting your stem cells over at least two passages and using adding Rock inhibitor (such as Y-27632). If adaptation proves challenging, consider using a higher concentration of Laminin 521 protein (e.g. 10 μg/ml). Once your stem cells are well adapted after several passages, you can gradually reduce the working concentration and cell density.

How do I passage my stem cell on Laminin 521 coating?

To passage iPSCs on Laminin 521-coated surfaces, first ensure that the coating is prepare according to the manufacturers’s instructions. Dissociate the cells using enzymes, like EDTA, Accutase, TrypLE Select. Typically, the incubation time for dissociation is approximately 5 to 6 minutes, though it may vary based on the specific cell line. Laminin 521 often results in  tighter cell attachment compared to other matrix proteins, so using a lower working concentration of Laminin 521 may help  reduce the detachment time.

How do I choose different kinds of laminin protein for stem cell expansion and differentiation?

Laminin proteins play a key role in stem cell expansion and differentiation due to their diverse tissue-specific expression . For hPSC culture, Laminin 521 and Laminin 511 are ideal for the maintaining high-quality stem cells. For differentiation into hepatic and neural cell, Laminin 111 and Laminin 121 are recommended. Laminin 211  supports differentiation into skeletal muscle, neural and cardiac cells while, Laminin 411 is suitable for endothelial and pancreatic cells differentiation.

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