Human IL-5 R alpha / CD125 Protein, His Tag, low endotoxin (MALS verified)

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Cat. No. / Size
Price
Qty
ILA-H52H4-50ug
$325.00
ILA-H52H4-1mg (250ug X 4)
$2470.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    IL5Ra, CD125

  • Source

    Human IL-5 R alpha, His Tag (ILA-H52H4) is expressed from human 293 cells (HEK293). It contains AA Asp 21 - Glu 335 (Accession # NP_000555).

    Predicted N-terminus: Asp 21

    Request for sequence
  • Molecular Characterization

    IL-5 R alpha Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 37.7 kDa. The protein migrates as 55-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    IL-5 R alpha SDS-PAGE

    Human IL-5 R alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IL-5 R alpha SEC-MALS

    The purity of Human IL-5 R alpha, His Tag (Cat. No. ILA-H52H4) is more than 95% and the molecular weight of this protein is around 43-63 kDa verified by SEC-MALS.

    Report
  • Bioactivity-BLI

     IL-5 R alpha BLI

    Loaded Biotinylated Human IL-5, His,Avitag (Cat. No. IL5-H82Q5) on SA Biosensor, can bind Human IL-5 R alpha, His Tag (Cat. No. ILA-H52H4) with an affinity constant of 12.6 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

    Protocol

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Background

Interleukin-5 receptor subunit alpha (IL5RA) is also known as IL-5 receptor subunit alpha, IL-5R subunit alpha and CD125,which is a single-pass type I membrane protein that belongs to the type I cytokine receptor family and type 5 subfamily.IL5R, a heterodimer of an alpha and a beta subunit, is expressed on eosinophils and basophils. IL5RA / CD125 is the receptor for interleukin-5 (IL5) and the alpha chain can bind to IL5. The WSXWS motif of IL5RA appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.The beta subunit of IL5R is common to the IL3, IL5 and GM-CSF receptors.

Recent Advances

 
Drug Development Progress
  • English Name:

    Interleukin-5 receptor subunit alpha

  • Category:

  • Approved Drugs:

    1 Details

  • Drugs in Clinical Trials:

    2 Details

  • Highest Development Stage:

    Approved

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