TIGIT Signaling Axis

TIGIT (T cell immununo receptor with lg and ITIM domains) is a newly discovered immune checkpoint protein expressed on T cells and Natural Killer cells (NK). TIGIT is an inhibitory receptor which modulates the immune activity by competing with activating receptor CD226 (also known as DNAM-1) for ligand binding. This on and off switch of TIGIT & DNAM-1 parallels that of CTLA4 & CD28, which makes it a promising target for anti-tumor therapy.

CD155 (PVR) and CD112 (PVRL2 or Nectin-2) are co-ligands of TIGIT and DNAM-1, while CD113 (PVRL3 or Nectin-3) is only binding to TIGIT. The affinity between different ligands and receptors vary. The binding between TIGIT and CD155 is more robust than that between TIGIT and CD112 or CD113.

ACROBiosystems carries a large collection of recombinant proteins from TIGIT signaling axis. Please find detailed information below:

Bioactivity Data

Fig.1 FACS assay shows Biotinylated Human TIGIT, Fc,Avitag (Cat. No. TIT-H82F1) can bind to 293T cell overexpressing human CD155. The concentration of TIGIT is 0.03 μg/mL.

Protocol

Fig.2 FACS assay shows that Human TIGIT, Mouse IgG2a Fc tag, low endotoxin (Cat. No. TIT-H5253) can bind to 293T cell overexpressing human CD155. The concentration of TIGIT used is 0.03 μg/mL.

Protocol

Fig.3 Anti-hTIGIT antibody captured on CM5 chip via Anti-mouse antibodies surface can bind Human TIGIT, His Tag (Cat. No. TIT-H52H5) with an affinity constant of 1.28 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol

Fig.4 Serial dilutions of Anti-TIGIT Neutralizing antibody (Catalog # EP131-C04) (1:1 serial dilution, from 5 μg/mL to 0.01μg/mL) was added into TIGIT: Biotinylated CD155 binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

Protocol

TIGIT Product List

Cat. No.	Species	Product Description	Structure	Purity	Features
TIT-H82F1 (Biotin-labeled)		Biotinylated Human TIGIT Protein, Fc Tag, Avi Tag
TIT-C5223		Cynomolgus / Rhesus macaque TIGIT Protein
TIT-C5254		Cynomolgus / Rhesus macaque TIGIT Protein, Fc Tag
TIT-M52E6		Mouse TIGIT Protein, His Tag
TIT-M5257		Mouse TIGIT Protein, Fc Tag
TIT-R5258		Rat TIGIT Protein, Fc Tag
TIT-R5259		Rabbit TIGIT Protein, Fc Tag
TIT-H5253		Human TIGIT Protein, Mouse IgG2a Fc Tag, low endotoxin
TIT-H5254		Human TIGIT Protein, Fc Tag
TIT-C52H7		Canine TIGIT Protein, His Tag

Other Relevant Products

Molecule	Cat. No.	Species	Product Description	Structure	Purity	Features
CD155	CD5-H82F6		Biotinylated Human CD155 / PVR Protein, Fc, Avi Tag
	CD5-R52H5		Rhesus macaque CD155 / PVR Protein, His Tag
	CD5-H5254		Human CD155 / PVR Protein, Mouse IgG2a Fc Tag, low endotoxin
	CD5-M5254		Mouse CD155 / PVR Protein, Mouse IgG2a Fc Tag
	CD5-H82E3		Biotinylated Human CD155 / PVR Protein, His, Avitag™
	CD5-M82F7		Biotinylated Mouse CD155 / PVR Protein, Fc, Avitag™
	CD5-H5251		Human CD155 / PVR Protein, Fc Tag
	CD5-H5223		Human CD155 / PVR Protein, His Tag
CD112	CD2-H82F8		Biotinylated Human Nectin-2 / CD112 Protein, Fc, Avi Tag
	CD2-H5257		Human Nectin-2 / CD112 Protein, Mouse IgG2a Fc Tag
	PV2-H5253		Human Nectin-2 / CD112 Protein, Fc Tag
	PV2-H52E2		Human Nectin-2 / CD112 Protein, His Tag
CD113	PV3-H82F3		Biotinylated Human Nectin-3 / CD113 Protein, Fc, Avi Tag
	PV3-H5255		Human Nectin-3 / CD113 Protein, Fc Tag
	PV3-H52E4		Human Nectin-3 / CD113 Protein, His Tag
DNAM-1	DN1-H82F9		Biotinylated Human DNAM-1 / CD226 Protein, Fc, Avi Tag
	DN1-C52H9		Cynomolgus DNAM-1 / CD226 Protein, His Tag
	DN1-H5257		Human DNAM-1 / CD226 Protein, Fc Tag
	DN1-H52H6		Human DNAM-1 / CD226 Protein, His Tag

Customer Reviews

References

• Yu X, et al., 2009, Nat. Immunol. 10:48-57.

• Stengel KF, et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109:5399-5404.

• Lozano E, et al., 2012, J. Immunol. 188:3869-3875.

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