ログイン 会員登録
icon_bulk_order一括注文 Acrobiosystems for English
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
phone
Your Position: ホーム > CelThera™ GMP T Cell Expansion Medium

Chemically Defined AOF No Serum or SR Needed Fast Cell Expansion

High Quality Laminin Protein supports Clinical iPSC Banking

T lymphocytes play a crucial role in the human immune system, and utilizing the inherent functions of specific T cells to combat diseases has become a breakthrough technological approach. T cell culture medium provides the nutritional matrix required for T cell proliferation. By simulating the in vivo microenvironment, isolated T cells can expand effectively in vitro while maintaining their structure and function. The development of T cell culture media has progressed through three important stages, among these, chemically defined media have become the preferred choice, as they ensure batch-to-batch consistency, traceability, and product stability, becoming the mainstream choice in the future.

High Quality Laminin Protein supports Clinical iPSC Banking
Development stages of T cell culture medium

CelThera™ GMP T Cell Expansion Medium

ACROBiosystems’ newly launched CelThera™ GMP T Cell Expansion Medium used an innovative chemical limited formula, without serum and animal derived component, focusing on improving the stability and controllability of T cell culture, helping to enhance T cell proliferation and vitality, and improving overall production efficiency.

Chemically defined, animal-origin free (AOF) and high batch-to-batch consistency.

No additional serum or serum replacement required to maintain optimal expansion rates.

Enables high fold expansion of T cells in a reduced timeframe (100-fold expansion in < 7 days, 3,000-fold expansion in 13 days).

Achieves a balanced CD4+: CD8+ 1:1 T-cell ratio more quickly.

Faster promotion of TN/SCM ratio increase in cells.

Request Custom Services

Promote rapid expansion of T cells, CAR-T cells, TCR-T cells, peripheral blood lymphocytes (PBL) and other T source cells.

Support scaling up from preclinical development to production.

Produced under GMP conditions.

Provide complete declaration support documents, complete set of methodological validation reports and other documents, FDA DMF filed.

Click to apply for RSF file.

Fast Expansion, Large-Scale Manufacturing Support!

Cultivation Scheme for PBMC-Derived T Cells in 6-Well Plate System Click for details

Cultivation Scheme for PBMC-Derived T Cells in 6-Well Plate System

Human PBMCs were cultured with GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) with CelThera™ GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3101) or T cell culture medium (Competitor L +2.5% SR) for two weeks. The result shows that CelThera™ GMP T Cell Expansion Medium (ACROBiosystems) can be comparable with the Competitor L +2.5% SR. Notably, the cells exhibits better expansion in CelThera™ GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3101).

Cultivation Scheme for PBMC-Derived T Cells in 6-Well Plate System

Human PBMCs were cultured with CelThera™ GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3101) or T cell culture medium (Competitor L +2.5% SR) for two weeks. The result shows that CelThera™ GMP T Cell Expansion Medium (ACROBiosystems) can be comparable with the Competitor L +2.5% SR.

Cultivation Scheme for PBMC-Derived T Cells in T225 Bottle Systems Click for details

Cultivation Scheme for PBMC-Derived T Cells in T225 Bottle Systems
Cultivation Scheme for PBMC-Derived T Cells in T225 Bottle Systems
Cultivation Scheme for PBMC-Derived T Cells in T225 Bottle Systems

Human PBMCs were cultured with GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) with CelThera™ GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3101 & GMP-CM3101-1) for two weeks. The result shows that CelThera™ GMP T Cell Expansion Medium with GMP Human IL-2 Protein can promote the expansion of these cells with a reasonable cell viability.

Cultivation Scheme for PBMC-Derived T Cells in T225 Bottle Systems
Cultivation Scheme for PBMC-Derived T Cells in T225 Bottle Systems
Cultivation Scheme for PBMC-Derived T Cells in T225 Bottle Systems

Human PBMCs were cultured with GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) with CelThera™ GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3101 & GMP-CM3101-1) for two weeks. The result shows that CelThera™ GMP T Cell Expansion Medium with GMP Human IL-2 Protein can increase the percentage of the CD3+ cells. With the post-culture time, the proportion of CD4 cells gradually decreased, and the proportion of CD8 cells gradually increased.

Cultivation Scheme of PBMC-Derived T Cells in 3L Systems Click for details

Cultivation Scheme of PBMC-Derived T Cells in 3L Systems
Cultivation Scheme of PBMC-Derived T Cells in 3L Systems
Cultivation Scheme of PBMC-Derived T Cells in 3L Systems

Human PBMCs were activated using 0.2 µg/mL GMP Monoclonal Anti-Human CD3 Antibody (OKT3) (ACROBiosystems, Cat. No. GMP-MC0323) and 1 µg/mL GMP Monoclonal Anti-Human CD28 Antibody (ACROBiosystems, Cat. No. GMP-MC2824), cultured with CelThera™ GMP T Cell Expansion culture medium (ACROBiosystems, Cat. No. GMP-CM3101) supplemented with 500 IU/mL GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) for two weeks. The results showed that GMP human IL-2 protein, GMP monoclonal anti-human CD3 antibody (OKT3), GMP monoclonal anti-human CD28 antibody and CelThrea™ GMP T cell expansion medium could be used to culture T cells in a 3L large system. It can efficiently expand cells with high viability.

Cultivation Scheme of PBMC-Derived T Cells in 3L Systems
Cultivation Scheme of PBMC-Derived T Cells in 3L Systems

Cell ratio of the human various T cells. Human PBMCs were activated using 0.2 µg/mL GMP Monoclonal Anti-Human CD3 Antibody (OKT3) (ACROBiosystems, Cat. No. GMP-MC0323) and 1 µg/mL GMP Monoclonal Anti-Human CD28 Antibody (ACROBiosystems, Cat. No. GMP-MC2824), and cultured in CelThera™ GMP T Cell Expansion culture medium (ACROBiosystems, Cat. No. GMP-CM3101) supplemented with 500 IU/mL GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) for two weeks. The result shows that percentage of CD3+ cells in living cells is above 99%. At the end of the culture process, the majority memory phenotype of T cells are TN/SCM, followed by TCM, which means that the cell harvest will have a promising effect, such as sustained proliferation, prolonged survival, and so on.

Frequently Asked Questions

What is the GMP-CM3101-1?  

GMP-CM3101-1 is our GMP T Cell Expansion Medium supplement, formulated without serum.

Does the culture medium contain factors or antibodies?

The base culture medium does not contain any growth factors or antibody components. These should be added separately, according to your experimental requirements.

Stability of GMP-CM3101 and GMP-CM3101-1 components after mixing.

After mixing two components, they can be stored at 4 ℃ for 1 month, but it is recommended to use them up within 2 weeks as much as possible after mixing.

How to thaw and use GMP-CM3101-1?

To use GMP-CM310-1, thaw it at room temperature. Once thawed, transfer the entire contents of the GMP-CM310-1 vail into the GMP-CM3101 base medium. Mix thoroughly before use.

ACRO Quality

This web search service is supported by Google Inc.

totopphone