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Your Position: ホーム > IgG Isotype Controls: Anti-DNP Antibodies

IgG Isotype Controls: Anti-DNP Antibodies

IgG Isotype Controls: Anti-DNP Antibodies
Anti-DNP Antibodies Introduction

Antibody drugs have become an important field in the development of biomedicine. Isotype control antibodies in antibody drug development are of the same class and subtype as the primary antibody developed against the target, but do not specifically bind to a known or potential target. The purpose of using the isotype control is to confirm that the binding or effect of the primary antibody is specific, excluding non-specific interactions with Fc receptor proteins or with other proteins. Meanwhile, isotype control can also be used as an antibody that competes for binding to Fc receptor proteins, which is related to functional blocking antibody perform the same function, which facilitates effective and reasonable evaluation of the efficacy of antibody drugs. Non-specific background is an important variable that needs to be controlled. Setting the corresponding isotype control antibody to detect non-specific binding can reduce the generation of false positive results and evaluate the possible influencing factors accurately in the drug development process.

DNP (2,4-dinitrophenol) is a hapten that induces a strong immune response when attached to a protein or polypeptide. DNP is absent in animal tissues. Therefore, anti-DNP antibodies are excellent isotype controls for antibodies without endogenous background effects. ACROBiosystems has developed a series of anti-DNP monoclonal antibodies with Human and Mouse species and different subtypes, which can be used as isotype control antibodies in various applications such as ELISA, western blot, immunofluorescence, immunohistochemistry (IHC) and flow cytometry (FACS), etc.

Anti-DNP Antibodies Product List
MoleculeCat. No.SpeciesProduct DescriptionPreorder/Order
Anti-DNP AntibodiesDNP-M2HumanMonoclonal Anti-DNP antibody, Human IgG1 Isotype Control (carrier free, MALS verified)

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DNP-BM2Biotinylated Monoclonal Anti-DNP antibody, Human IgG1 Isotype Control (carrier free, MALS verified)

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DNP-MB273Monoclonal Anti-DNP antibody, Human IgG1 (N297A)   Isotype Control (carrier free, MALS verified)

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DNP-M915Monoclonal Anti-DNP antibody, Human IgG3 Isotype Control (carrier free)

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DNP-M3Monoclonal Anti-DNP antibody, Human IgG4 Isotype Control (carrier free, MALS verified)

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DNP-FM2FITC-Labeled Monoclonal Anti-DNP antibody, Human IgG1 Isotype Control (carrier free)

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DNP-M1MouseMonoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control (Carrier free, MALS verified)

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DNP-BM1Biotinylated Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control   (carrier free, MALS verified)

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DNP-M1A1Monoclonal Anti-DNP   antibody, Mouse IgG1 Isotype Control

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DNP-BM190Biotinylated Monoclonal Anti-DNP antibody, Mouse IgG1   Avitag™ Isotype Control

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DNP-FM1FITC-Labeled Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control   (carrier free)

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DNP-FM1A1FITC-Labeled Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control

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DNP-PM1PE-Labeled Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control (Site-specific conjugation)

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Anti-DNP Antibodies Verification Data

High purity and structural homogeneity verified by SDS-PAGE and SEC-MALS

purity_ACA-H421b

Monoclonal Anti-DNP antibody, Human IgG1 (N297A) Isotype Control on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%. The purity of Monoclonal Anti-DNP antibody, Human IgG1 (N297A) Isotype Control (Cat. No. DNP-MB273) is more than 90% and the molecular weight of this protein is around 140-160 kDa verified by SEC-MALS.

purity_GCF-H5214

Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%. The purity of Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control (Cat. No. DNP-M1) is more than 90% and the molecular weight of this protein is around 130-160 kDa verified by SEC-MALS.

High biological activity verified by ELISA

DNP-M2-E1

Immobilized DNP-Albumin Conjugate at 10 μg/mL (100 μL/well) can bind Monoclonal Anti-DNP antibody, Human IgG1 Isotype Control (Cat. No. DNP-M2) with a linear range of 1-10 ng/mL (QC tested).

DNP-M3-E1

Immobilized DNP-Albumin Conjugate at 10 μg/mL (100 μL/well) can bind Monoclonal Anti-DNP antibody, Human IgG4 Isotype Control (Cat. No. DNP-M3) with a linear range of 1-15 ng/mL (QC tested).

DNP-MB273-E1

Immobilized DNP-Albumin Conjugate at 10 μg/mL (100 μL/well) can bind Monoclonal Anti-DNP antibody, Human IgG1 (N297A) Isotype Control (Cat. No. DNP-MB273) with a linear range of 1-10 ng/mL (QC tested).

DNP-M1-E1

Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control (Cat. No. DNP-M1) with a linear range of 2-20 ng/mL (QC tested).

FACS verified for use as isotype controls

DNP-BM1-F3

2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1 μg/mL of Biotinylated Anti-DNP Antibody, Mouse IgG1 (Cat. No. DNP-BM1) and positive control respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).

DNP-FM1-F3

2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1 μg/mL of FITC-Labeled Anti-DNP Antibody, Mouse IgG1 (Cat. No. DNP-FM1) and positive control respectively, and FITC signal was used to evaluate the binding activity (Routinely tested).

DNP-M1A1-F1

2e5 of anti-CD19 CAR-293 cells were stained with 100ul of 1:50 dilution of Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control (Cat. No. DNP-M1A1) and positive control antibody respectively, washed and then followed by PE anti-mouse IgG1 and analyzed with FACS (QC tested).

DNP-BM190-F1

2e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) Biotinylated Monoclonal Anti-DNP antibody, Mouse IgG1 Avitag Isotype Control (Cat. No. DNP-BM190) and positive control antibody (Cat. No. FM3-BY54) respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).

DNP-FM1A1-F1

2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of FITC-Labeled Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control (Cat. No. DNP-FM1A1) and positive control (Cat. No. FM3-FY45P1) respectively, and FITC signal was used to evaluate the binding activity (QC tested).

DNP-PM1-F1

5e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control (Cat. No. DNP-PM1) and positive control (Cat. No. FM3-HPY53) respectively, and PE signal was used to evaluate the binding activity (QC tested).

Other Isotype Controls

IgG Fc Proteins——Ideal Isotype Control for IgG Structured Drug Development

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