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Q1: What should I do upon receiving live organoids?

Upon receiving live organoids, you should first culture them in the recovery medium for a period of time (please refer to the protocol included with the product for specific instructions). After this initial recovery phase, you can then transfer the organoids to the normal maintenance medium for ongoing culture and experimentation.

Q2:How do I ensure successful organoid growth using the differentiation kit?

We will provide detailed protocol instructions and step-by-step explanatory videos. If reliable iPSC lines are used, the success rate should be high. If you encounter failure in your first attempt, please contact us and we will help you to troubleshoot and help you succeed in growing your own organoids.

Q3: On which day can the cardiac organoid be considered as mature?

The cardiac organoid is considered mature at 21 days in vitro.

Q4: On which day can the cerebral organoid be determined as a mature brain?

Cerebral organoid cell types are considered mature at 90 days in vitro. However, for the maturity of the electrophysiological activity, the cerebral organoids need to be cultured for at least 120 days. This indicates that while the cell types may reach a certain level of maturity earlier, the development of more complex neural functions, such as electrophysiological activity, requires additional time.

Q5:Compared to Matrigel culture, what advantage does ACROBiosystems method have?

Matrigel can expedite the early phases of organoid culture, but it is derived from tumor extracellular matrix (ECM) which is a non-physiological environment. In Matrigel cultures, cells tend to migrate to the outer layer, which can lead to the formation of a dead core. Additionally, Matrigel hinders extracellular electrophysiological measurements due to its lower conductivity compared to actual tissue. Our protocol, on the other hand, enables the formation of the organoid's own ECM, simulating the natural developmental process. Our method aims to address the issues with Matrigel and promote more physiologically relevant conditions for organoid growth and study.

Q6: Can ACROBiosystems's organoids be passaged? What is the difference between passable and non-passable organoids?

We currently offer passage-able liver and intestinal organoids. Passage-able organoids refer to those that can be successfully propagated or passaged, meaning they can be cultured for multiple generations. These organoids typically have simpler cell lineages and fewer cell types compared to non-passable organoids. Non-passage-able organoids, on the other hand, may not be suitable for repeated propagation due to their more complex structure or specific cell requirements.

Q7: Where can I find the maintenance and culture methods/requirements for cerebral organoids?

You can find the maintenance and culture methods/requirements for cerebral organoids on our webpage dedicated to cerebral organoids or the maintenance and maturation media kit. On this page, please select the DS file, which contains the protocol for culturing the organoids as well as the requirements for lab equipment.

Q8: What are the methods that ACROBiosystems uses to keep organoids alive during the transport process?

During the transport process, ACROBiosystems uses a recovery medium with a high concentration of growth factors and specific chemical compounds to prevent cell death. It is also crucial to work in a sterile environment since the medium does not contain antibiotics. These measures help ensure the viability of the organoids during shipping.

Q9: How does the "vasculature" look like in organoids? Are there functional vascular tubes?

In organoids, the "vasculature" manifests as an endothelial cell network. While these networks are formed by the interaction of endothelial cells, they do not constitute functional vascular tubes. The endothelial cell network plays a crucial role in supporting the growth and development of the organoids, but it does not fully replicate the complex vascular system found in native tissues.

Q10: What disease models can ACROBiosystems currently develop?

ACROBiosystems can currently develop models for Alzheimer's, Parkinson's, heart failure, and inflammatory bowel disease using organoids. Custom models are also available upon request.

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