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Upon receiving live organoids, you should first culture them in the recovery medium for a period of time (please refer to the protocol included with the product for specific instructions). After this initial recovery phase, you can then transfer the organoids to the normal maintenance medium for ongoing culture and experimentation.

We will provide detailed protocol instructions and step-by-step explanatory videos. If reliable iPSC lines are used, the success rate should be high. If you encounter failure in your first attempt, please contact us and we will help you to troubleshoot and help you succeed in growing your own organoids.

The cardiac organoid is considered mature at 21 days in vitro.

Cerebral organoid cell types are considered mature at 90 days in vitro. However, for the maturity of the electrophysiological activity, the cerebral organoids need to be cultured for at least 120 days. This indicates that while the cell types may reach a certain level of maturity earlier, the development of more complex neural functions, such as electrophysiological activity, requires additional time.

Matrigel can expedite the early phases of organoid culture, but it is derived from tumor extracellular matrix (ECM) which is a non-physiological environment. In Matrigel cultures, cells tend to migrate to the outer layer, which can lead to the formation of a dead core. Additionally, Matrigel hinders extracellular electrophysiological measurements due to its lower conductivity compared to actual tissue. Our protocol, on the other hand, enables the formation of the organoid's own ECM, simulating the natural developmental process. Our method aims to address the issues with Matrigel and promote more physiologically relevant conditions for organoid growth and study.

You can find the maintenance and culture methods/requirements for cerebral organoids on our webpage dedicated to cerebral organoids or the maintenance and maturation media kit. On this page, please select the DS file, which contains the protocol for culturing the organoids as well as the requirements for lab equipment.

During the transport process, ACROBiosystems uses a recovery medium with a high concentration of growth factors and specific chemical compounds to prevent cell death. It is also crucial to work in a sterile environment since the medium does not contain antibiotics. These measures help ensure the viability of the organoids during shipping.

In organoids, the "vasculature" manifests as an endothelial cell network. While these networks are formed by the interaction of endothelial cells, they do not constitute functional vascular tubes. The endothelial cell network plays a crucial role in supporting the growth and development of the organoids, but it does not fully replicate the complex vascular system found in native tissues.

ACROBiosystems can currently develop models for Alzheimer's, Parkinson's, heart failure, and inflammatory bowel disease using organoids. Custom models are also available upon request.