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Human CD4 / LEU3 Protein, His Tag (MALS verified) DMF

  • Synonym
    CD4,CD4mut,LEU3
  • Source
    Human CD4, His Tag(LE3-H5228) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Trp 390 (Accession # AAH25782).
    Predicted N-terminus: Lys 26
  • Molecular Characterization
    CD4 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 41.5 kDa. The protein migrates as 47-54 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD4 SDS-PAGE

Human CD4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
CD4 MALS images

The purity of Human CD4, His Tag (Cat. No. LE3-H5228) is more than 95% and the molecular weight of this protein is around 40-50 kDa verified by SEC-MALS.

Bioactivity-ELISA
 CD4 ELISA

Immobilized Ibalizumab at 1 μg/mL (100 μL/well) can bind Human CD4, His Tag (Cat. No. LE3-H5228) with a linear range of 0.8-6 ng/mL (QC tested).

 CD4 ELISA

Immobilized Human CD4, His Tag (Cat. No. LE3-H5228) at 1 μg/mL (100 μL/well) can bind Ibalizumab with a linear range of 0.2-13 ng/mL (Routinely tested).

 CD4 ELISA

Immobilized Human CD4, His Tag (Cat. No. LE3-H5228) at 2 μg/mL (100 μL/well) can bind Anti-CD4 with a linear range of 0.4-7 ng/mL (Routinely tested).

  • Background
    T-cell surface glycoprotein CD4 is also known as T-cell surface antigen T4/Leu-3. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is accessory protein for MHC class-II antigen/T-cell receptor interaction. CD4 induces the aggregation of lipid rafts. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Therefore, medical professionals refer to the CD4 count to decide when to begin treatment for HIV-infected patients.
  • Clinical and Translational Updates

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価格(JPY) : 420,000

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