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Your Position: ホーム > Protein > IgG3 Fc > IG3-H5200

Human IgG3 Fc Protein, Tag Free (MALS & SPR verified)

  • シノニム
    Ig gamma-3 chain C region,IgG,IgG3
  • ソース
    Human IgG3 Fc, Tag Free (IG3-H5200) is expressed from human 293 cells (HEK293). It contains AA Glu 99 - Lys 377 (Accession # P01860).
    Predicted N-terminus: Glu 99
  • 分子特性
    Online(Glu 99 - Lys 377) P01860

    This protein carries no "tag".

    The protein has a calculated MW of 31.2 kDa. The protein migrates as 35-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • エンドトキシン
    Less than 1.0 EU per μg by the LAL method.
  • 純度

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 成分

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 再構成

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 保存

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human IgG3 Fc, Tag Free (Cat. No. IG3-H5200) SDS-PAGE gel

Human IgG3 Fc, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human IgG3 Fc, Tag Free (Cat. No. ) MALS images

The purity of Human IgG3 Fc, Tag Free (Cat. No. IG3-H5200) is more than 90% and the molecular weight of this protein is around 58-71 kDa verified by SEC-MALS.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCN-H52W7) captured on CM5 Chip via anti-His antibody can bind Human IgG3 Fc, Tag Free (Cat. No. IG3-H5200) with an affinity constant of 2.82 μM as determined in SPR assay (Biacore 8K) (QC tested).

  • バックグラウンド
    Immunoglobulin G3 (IgG3) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-3 chain C region (IgG3-Fc / IGHG3) contains two constant regions of IgG3 H chain (CH2, CH3).
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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ETD of in-stock products: 4 business days

価格(JPY) : 75,600

価格(JPY) : 201,600

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