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Your Position: ホーム > Protein > B7-2 > CD6-C5254

Cynomolgus / Rhesus macaque B7-2 / CD86 Protein, Fc Tag

  • シノニム
    CD86,B7-2,B70,CD28LG2,LAB72,MGC34413
  • ソース
    Cynomolgus / Rhesus macaque B7-2, Fc Tag (CD6-C5254) is expressed from human 293 cells (HEK293). It contains AA Ala 19 - His 240 (Accession # G7NXR4). In the region Ala 19 - His 240, the AA sequence of Cynomolgus and Rhesus macaque B7-2 are homologus.
    Predicted N-terminus: Ala 19
  • 分子特性
    Online(Ala 19 - His 240) G7NXR4

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 52 kDa. The protein migrates as 76-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • エンドトキシン
    Less than 1.0 EU per μg by the LAL method.
  • 純度

    >95% as determined by SDS-PAGE.

  • 成分

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 再構成

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 保存

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus / Rhesus macaque B7-2, Fc Tag (Cat. No. CD6-C5254) SDS-PAGE gel

Cynomolgus / Rhesus macaque B7-2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Cynomolgus / Rhesus macaque B7-2, Fc TagCynomolgus / Rhesus macaque B7-2, Fc Tag (Cat. No. CD6-C5254) ELISA bioactivity

Immobilized Cynomolgus / Rhesus macaque CTLA-4, His Tag (Cat. No. CT4-C5227) at 2 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque B7-2, Fc Tag (Cat. No. CD6-C5254) with a linear range of 0.039-1.25 μg/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Cynomolgus / Rhesus macaque B7-2, Fc Tag (Cat. No. CD6-C5254) on Protein A Biosensor, can bind Human / Cynomolgus / Rhesus macaque CD28, His Tag (Cat. No. CD8-H52Hc) with an affinity constant of 11 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • バックグラウンド
    Cluster of Differentiation 86 (CD86) is also known as B-lymphocyte activation antigen B7-2, is a type I membrane protein that is a member of the immunoglobulin superfamily, and is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B72 is expressed at low levels on monocytes and can be upregulated through interferon γ. CD86 is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD86 works in tandem with CD80 to prime T cells. Recent study has revealed that B7-2 promotes the generation of a mature APC repertoire and promotes APC function and survival. Furthermore, the B7 proteins are also involved in innate immune responses by activating NF-κB-signaling pathway in macrophages. CD86 thus is regarded as a promising candidate for immune therapy. CD86+ macrophages in Hodgkin lymphoma patients are an independent marker for potential nonresponse to firstline-therapy.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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価格(JPY) : 492,800

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