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Your Position: ホーム > Protein > G-CSF R > GCR-H82E4

Biotinylated Human G-CSF R / CD114 Protein, Avitag™,His Tag (MALS verified)

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    CSF3R,CD114,GCSFR
  • Source
    Biotinylated Human G-CSF R, Avitag,His Tag(GCR-H82E4) is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Pro 621 (Accession # NP_000751).
    Predicted N-terminus: Glu 25
    Request for sequence
  • Molecular Characterization
    G-CSF R Structure

    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag

    The protein has a calculated MW of 69.2 kDa. The protein migrates as 85-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
G-CSF R SDS-PAGE

Biotinylated Human G-CSF R, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
G-CSF R MALS images

The purity of Biotinylated Human G-CSF R, Avitag,His Tag (Cat. No. GCR-H82E4) is more than 85% and the molecular weight of this protein is around 148-184 kDa verified by SEC-MALS.

Report
Bioactivity-ELISA
G-CSF R ELISA

Immobilized Human G-CSF Protein, premium grade (Cat. No. GCF-H5214) at 2 μg/mL (100 μL/well) can bind Biotinylated Human G-CSF R, Avitag,His Tag (Cat. No. GCR-H82E4) with a linear range of 0.01-0.6 μg/mL (QC tested).

Protocol
  • Background
    Granulocyte Colony Stimulating Factor Receptor (G-CSFR) is also known as Cluster of Differentiation 114 (CD114), CSF3R and GCSF, is a cell-surface receptor for the granulocyte colony-stimulating factor (G-CSF), a cytokine that plays a critical role in the regulation of the activation, proliferation, differentiation, and survival of the neutrophilic granulocyte lineage. G-CSFR belongs to a family of cytokine receptors known as the hematopoietin receptor family. This type I  membrane protein has a composite structure consisting of an immunoglobulin(Ig)-like domain, a cytokine receptor-homologous (CRH) domain and three fibronectin type I II (FNIII) domains in the extracellular region. G-CSFR is present mainly on precursor cells in the bone marrow, and, in response to stimulation by G-CSF, initiates cell proliferation and differentiation into mature neutrophilic granulocytes and macrophages. G-CSFR mediates the specific effect of GCSF through activating a variety of intracellular signaling cascades, including the Jak/Stat, PI3/Akt, Ras-Raf-MAP kinase, and Src family kinase pathways, and thus functions in defense against infection, inflammation and repair, and in the maintenance of steady state hematopoiesis. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia. Mutations in the intracellular part of this receptor are also associated with certain types of leukemia.
  • Clinical and Translational Updates

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