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Biotinylated Human OX40 / TNFRSF4 / CD134 Protein, Avitag™,His Tag (MALS verified) (recommended for biopanning)

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.

  • シノニム
    TNFRSF4,OX40,CD134,OX40L receptor,ACT35,TXGP1L
  • ソース
    Biotinylated Human OX40, Avitag,His Tag (TN4-H82E4) is expressed from human 293 cells (HEK293). It contains AA Leu 29 - Ala 216 (Accession # NP_003318.1).
    Predicted N-terminus: Leu 29
  • 分子特性
    Online(Leu 29 - Ala 216) NP_003318.1

    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 22.8 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • ビオチン化
    ビオチン化 of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • エンドトキシン
    Less than 1.0 EU per μg by the LAL method.
  • 純度

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 成分

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 再構成

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 保存

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human OX40, Avitag,His Tag (Cat. No. TN4-H82E4) SDS-PAGE gel

Biotinylated Human OX40, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human OX40, Avitag,His Tag (Cat. No. ) MALS images

The purity of Biotinylated Human OX40, Avitag,His Tag (Cat. No. TN4-H82E4) is more than 90% and the molecular weight of this protein is around 35-50 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human OX40, Avitag,His TagBiotinylated Human OX40, Avitag,His Tag (Cat. No. TN4-H82E4) ELISA bioactivity

Immobilized Anti-OX40 MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human OX40, Avitag,His Tag (Cat. No. TN4-H82E4) with a linear range of 1-5 ng/mL (QC tested).

Biotinylated Human OX40, Avitag,His TagBiotinylated Human OX40, Avitag,His Tag (Cat. No. TN4-H82E4) ELISA bioactivity

Immobilized Biotinylated Human OX40, Avitag,His Tag (Cat. No. TN4-H82E4) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Human OX40 Ligand, Fc Tag (Cat. No. OXL-H526x) with a linear range of 0.2-10 ng/mL (Routinely tested).

Biotinylated Human OX40, Avitag,His TagBiotinylated Human OX40, Avitag,His Tag (Cat. No. TN4-H82E4) ELISA bioactivity

Immobilized Human CTLA-4, His Tag, active dimer (Cat. No.CT4-H52H9) at 2 μg/mL, add increasing concentrations of CTLA-4 x OX40 Bispecific Antibody in 50% Human serum and then add Biotinylated Human OX40, Avitag,His Tag(Cat. No. TN4-H82E4) at 1 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4 ng/mL (Intact assay, Routinely tested).

Biotinylated Human OX40, Avitag,His TagBiotinylated Human OX40, Avitag,His Tag (Cat. No. TN4-H82E4) ELISA bioactivity

Immobilized Biotinylated Human OX40, Avitag,His Tag (Cat. No. TN4-H82E4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-OX40 Antibody, Human IgG2 with a linear range of 0.1-4 ng/mL (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Human OX40, Avitag,His Tag (Cat. No. TN4-H82E4) on SA Biosensor, can bind Anti-OX40 MAb (Human IgG1) with an affinity constant of 0.115 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Human OX40, Avitag,His Tag (Cat. No. TN4-H82E4) on SA Biosensor, can bind Human OX40 Ligand, His Tag (active trimer) (MALS verified) (Cat. No. OXL-H52Q8) with an affinity constant of 0.127 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Citations
  • バックグラウンド
    Tumor necrosis factor receptor superfamily member 4 (TNFRSF4) is also known as ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, CD antigen CD134, OX40. OX40 / TNFRSF4 contains four TNFR-Cys repeats. TNFRSF4 is receptor for TNFSF4 / OX40L / GP34 and can interacts with TRAF2, TRAF3 and TRAF5.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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